Abstract
Introduction
The selection of the right IBD control strategy is primarily based on the choice of the appropriate vaccine strain. High maternal IBD-specific antibodies (Abs) compete with the efficacy IBD vaccine, which necessitates the application of intermediate-plus vaccine strain.
Methods
A comparative experimental study was designed for evaluation of four different commercially available intermediate-plus IBD vaccines in commercial broilers before complete weaning of IBD-specific maternal Abs.
Results
As determined by IBD- specific quantitative real-time polymerase chain reaction, three tested vaccine strains (228E, Winterfield H2512, and Winterfield 2512) were able to establish in the bursal tissues as early as six hours (hrs) post-vaccination (PV). Both the 228E and the Winterfield H2512 strains vaccinated groups had the highest viral load and replication rate in the bursal tissues at 24, 36, 48 and 72 hrs PV. Earlier seroconversion, 7-14 days PV, was observed in the case of Winterfield H2512, 228E, and Winterfield 2512 vaccinated birds compared to the Lukert vaccinated birds. The 228E strain was more virulent and induces the highest lesion score with severe degrees of lymphocyte depletion and necrosis which persisted up to 28 days PV.
Conclusion
Overall, the different intermediate-plus IBD strains possess variable early kinetics in the bursal tissues and eliciting antibody (Ab) responses differently withdifferent degrees of bursal lesions. The assessment of the intrabursal vaccine load together with humoral immunity and bursal damage lesion score are fundamental parameters in the evaluation of the intermediate-plus IBD vaccines.
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Data availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
References
Barlic-Maganja D, Zorman-Rojs O, Grom J (2002) Detection of infectious bursal disease virus in different lymphoid organs by single-step reverse transcription polymerase chain reaction and microplate hybridization assay. J Vet Diagn Investig 14:243–246. https://doi.org/10.1177/104063870201400310
Cloud SS, Lillehoj HS, Rosenberger JK (1992) Immune dysfunction following infection with chicken anemia agent and infectious bursal disease virus. I Kinetic alterations of avian lymphocyte subpopulations. Vet Immunol Immunopathol 34:337–352. https://doi.org/10.1016/0165-2427(92)90174-o
de Wit JJ (1998) Gumboro disease: estimation of optimal time of vaccination by the Deventer formula. Pol Vet J 3:19–22
Delmas B, Attoui H, Ghosh S, Malik YS, Mundt E, Vakharia VN, Ictv Report C (2019) ICTV virus taxonomy profile: Birnaviridae. J Gen Virol 100:5–6. https://doi.org/10.1099/jgv.0.001185
Eterradossi N, Saif YM (2013) Infectious bursal disease. In: Swayne DE (ed) Diseases of poultry, 13th edn. John Wiley & Sons, Inc, pp 219–246
Gelb J Jr, Jackwood DJ, Brannick EM, Ladman BS (2016) Efficacy of recombinant HVT-IBD vaccines administered to broiler chicks from a single breeder flock at 30 and 60 weeks of age. Avian Dis 60:603–612. https://doi.org/10.1637/11344-120815-Reg.1
Gibson-Corley KN, Olivier AK, Meyerholz DK (2013) Principles for valid histopathologic scoring in research. Vet Pathol 50:1007–1015
Güneysu E, Kocman AE, Özatik O, Ovali C, Can B, Alataş İÖ, Sevin MB (2017) The effects of iloprost and alprostadil on ischemia-reperfusion injury in preventing inflammation, tissue degeneration, and apoptosis in rat skeletal muscle. Turk J Med Sci 47:1028–1036
Hitchner SB (1970) Infectivity of infectious bursal disease virus for embryonating eggs. Poult Sci 49:511–516. https://doi.org/10.3382/ps.0490511
Jakka P, Reddy YK, Kirubaharan JJ, Chandran ND (2014) Evaluation of immune responses by live infectious bursal disease vaccines to avoid vaccination failures. Eur J Microbiol Immunol 4:123–127. https://doi.org/10.1556/EuJMI.4.2014.2.5
Jayasundara J, Walkden-Brown SW, Islam A, Katz ME, Renz KG (2018) Tissue distribution, shedding and environmental detection of infectious bursal disease virus genome following infection of meat chickens at two ages. Australian Veterinary Journal 96:167–175. https://doi.org/10.1111/avj.12691
Kibenge FS, Dhillon AS, Russell RG (1988) Biochemistry and immunology of infectious bursal disease virus. J Gen Virol 69(Pt 8):1757–1775. https://doi.org/10.1099/0022-1317-69-8-1757
Killian MP, Boviez JD, Gambarotta M, Lombardo DM (2017) Induction of apoptosis in the bursa of Fabricius by vaccination against Gumboro disease. Avian Pathol 46:526–534. https://doi.org/10.1080/03079457.2017.1322684
Kurukulasuriya S et al (2017) Modified live infectious bursal disease virus (IBDV) vaccine delays infection of neonatal broiler chickens with variant IBDV compared to Turkey herpesvirus (HVT)-IBDV vectored vaccine. Vaccine 35:882–888. https://doi.org/10.1016/j.vaccine.2017.01.005
Le Gros FX, Dancer A, Giacomini C, Pizzoni L, Bublot M, Graziani M, Prandini F (2009) Field efficacy trial of a novel HVT-IBD vector vaccine for 1-day-old broilers. Vaccine 27:592–596. https://doi.org/10.1016/j.vaccine.2008.10.094
Lucio B, Hitchner SB (1979) Response of susceptible versus immune chicks to killed, live-modified, and wild infectious bursal disease virus vaccines. Avian Dis 23:1037–1050
Mazariegos LA, Lukert PD, Brown J (1990) Pathogenicity and immunosuppressive properties of infectious bursal disease “intermediate” strains. Avian Dis 34:203–208
Muller R, Kaufer I, Reinacher M, Weiss E (1979) Immunofluorescent studies of early virus propagation after oral infection with infectious bursal disease virus (IBDV). Zentralbl Veterinaermed [B] [ZDB] 26:345–352. https://doi.org/10.1111/j.1439-0450.1979.tb00823.x
Muller H, Mundt E, Eterradossi N, Islam MR (2012) Current status of vaccines against infectious bursal disease. Avian Pathol 41:133–139. https://doi.org/10.1080/03079457.2012.661403
Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS (2009) Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis 53:347–355. https://doi.org/10.1637/8456-082208-Reg.1
Prandini F, Simon B, Jung A, Poppel M, Lemiere S, Rautenschlein S (2016) Comparison of infectious bursal disease live vaccines and a HVT-IBD vector vaccine and their effects on the immune system of commercial layer pullets. Avian Path 45:114–125. https://doi.org/10.1080/03079457.2015.1127891
Rautenschlein S, Yeh HY, Sharma JM (2003) Comparative immunopathogenesis of mild, intermediate, and virulent strains of classic infectious bursal disease virus. Avian Dis 47:66–78. https://doi.org/10.1637/0005-2086(2003)047[0066:CIOMIA]2.0.CO;2
Rautenschlein S, Kraemer C, Vanmarcke J, Montiel E (2005) Protective efficacy of intermediate and intermediate plus infectious bursal disease virus (IBDV) vaccines against very virulent IBDV in commercial broilers. Avian Dis 49:231–237. https://doi.org/10.1637/7310-112204R
Rautenschlein S, von Samson-Himmelstjerna G, Haase C (2007) A comparison of immune responses to infection with virulent infectious bursal disease virus (IBDV) between specific-pathogen-free chickens infected at 12 and 28 days of age. Vet Immunol Immunopathol 115:251–260. https://doi.org/10.1016/j.vetimm.2006.11.002
Winterfield RW, Dhillon AS, Thacker HL (1981) Characteristics of apparent derivatives of the 2512 strain of infectious bursal disease virus when used as vaccines. Avian Dis 25:900–910
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Hamad, M., Hassanin, O., Ali, F.A.Z. et al. Comparative study on dynamic and immunopathology of four intermediate-plus infectious bursal disease (IBD) vaccines in commercial broiler chickens. Vet Res Commun 44, 147–157 (2020). https://doi.org/10.1007/s11259-020-09782-z
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DOI: https://doi.org/10.1007/s11259-020-09782-z