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Supplementation with long-acting injectable progesterone 3 days after TAI impaired luteal function in buffaloes

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Abstract

Three experiments were conducted to evaluate the effects of long-acting injectable progesterone (iP4) in buffalo cows. In Experiment 1, ovariectomized buffaloes received 300 mg (iP300) or 600 mg (iP600) of iP4, and serum P4 concentrations were evaluated. In experiment 2, three groups were compared: control or administration of 300 mg of iP4 3 (iP4-D3) or 6 days (iP4-D6) after timed artificial insemination (TAI). On day 16, reproductive tract was recovered for conceptus, endometrium, and corpus luteum (CL) analysis. In experiment 3, pregnancy per AI (P/TAI) and proportion of pregnancy losses were evaluated after administration of 300 mg of iP4 3 (iP4-D3) or 6 days (iP4-D6) after TAI in lactating buffaloes. In experiment 1, serum P4 concentrations remained over 1 ng/mL for ~ 3 days in both groups. The 300 mg dose was used in subsequent experiments. In experiment 2, CL weight and endometrial glands density were decreased, and conceptus length was increased in iP4-D3 compared to control and to iP4-D6 (P < 0.05). Transcript abundance of Prostaglandin F Receptor (FP) and ISG15 in CL and of ISG15 and MX1 in endometrium was greater in iP4-D3 when compared to control and to iP4-D6 (P < 0.05). In experiment 3, there was no difference among experimental groups for P/TAI at D30 and pregnancy losses (P > 0.1); however, iP4-D3 presented a lower P/TAI at day 60 (41.7%) when compared to control (56.8%) and iP4-D6 (57.7%; P = 0.07). In conclusion, administration iP4 at 3 days after TAI affects CL development and consequently decreases final pregnancy outcome in buffaloes.

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Data availability

Data will be made available on request.

Abbreviations

BCS:

Body condition score

CL:

Corpus Luteum

DCL:

Diameter of the CL

ESR1 :

Estrogen Receptor 1

FP :

Prostaglandin F Receptor

im:

Intramuscularly

INFt:

Interferon tau

iP300:

300 Mg of iP4

iP4:

Long-acting injectable progesterone

iP4-D3:

300 Mg of iP4 three days after timed-artificial insemination

iP4-D6:

300 Mg of iP4 six days after timed artificial insemination.

iP600:

600 Mg of iP4

ISG15 :

Interferon, Alpha-Inducible Protein Clone IFI-15 K

LLC:

Large luteal cells

MX1 :

Myxovirus Resistance 1 Interferon-Inducible

P/TAI:

Pregnancy per Artificial Insemination

P4:

Progesterone

PGF:

Prostaglandin f2 alpha

PGR :

Progesterone Receptor

PTGES :

Prostaglandin E Synthase

SLC:

Small luteal cells

TAI:

Timed artificial Insemination

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Funding

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) PDJ program (AMGD award number 150844/2017–4) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). The funders had no role in study design, data collection and analysis, decision to publish, or manuscript preparation.

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Author notes

  1. Diego Cavalcante de Souza and Angela Maria Gonella-Diaza and Nelcio Antonio Tonizza de Carvalho contributed equally to this work.

Authors and Affiliations

  1. Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil

    Diego Cavalcante de Souza, Flavia Morag Elliff, Júlia Gleyci Soares de Carvalho, Lais Mendes Vieira, Ed Hoffman Madureira, Guilherme Pugliesi & Pietro Sampaio Baruselli

  2. North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 3925 Hwy 75, Marianna, FL, 32446, USA

    Angela Maria Gonella-Diaza

  3. Registro Regional Research Center - Institute of Animal Science, Registro, SP, Brazil

    Nelcio Antonio Tonizza de Carvalho

  4. Department of Biological and Health Sciences, São Judas Tadeu University, Mooca, São Paulo, SP, Brazil

    Antenor P. Bonfim-Neto

  5. School of Veterinary Medicine, UniLaSalle Polytechnic Institute, Rouen, France

    Paula de Carvalho Papa

  6. Guru Angad Dev Veterinary &, Animal Sciences University, Ludhiana, Punjab, India

    Sarvpreet Singh Ghuman

  7. Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA

    Mario Binelli

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  1. Diego Cavalcante de Souza
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Contributions

Nelcio Antonio Tonizza de Carvalho, Pietro Sampaio Baruselli, Mario Binelli, Paula de Carvalho Papa, and Ed Hoffman Madureira designed the study protocol and reviewed the manuscript. Diego Cavalcante de Souza, Angela Maria Gonella-Diaza, Nelcio Antonio Tonizza de Carvalho, Júlia Gleyci Soares de Carvalho, Flavia Morag Elliff, Lais Mendes Vieira, Antenor Pereira Bonfim Neto, and Sarvpreet Singh Ghuman conducted the study and performed the laboratory analysis. Diego Cavalcante de Souza and Angela Maria Gonella-Diaza analyzed and interpreted the data and wrote the first version of the manuscript. Guilherme Pugliesi, Mario Binelli, and Nelcio Antonio Tonizza de Carvalho reviewed and improved the manuscript. All authors read and approved the final manuscript.

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Correspondence to Angela Maria Gonella-Diaza.

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Cavalcante de Souza, D., Gonella-Diaza, A.M., de Carvalho, N.A.T. et al. Supplementation with long-acting injectable progesterone 3 days after TAI impaired luteal function in buffaloes. Trop Anim Health Prod 56, 76 (2024). https://doi.org/10.1007/s11250-024-03913-3

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