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Effect of dietary supplemental vitamin C and betaine on the growth performance, humoral immunity, immune organ index, and antioxidant status of broilers under heat stress

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Abstract

Heat stress (HS) has become one of the important factors affecting the development of animal husbandry. The purpose of this experiment was to investigate whether vitamin C (Vc) and betaine (Bet) improve immune organ index and humoral immunity by enhancing the antioxidant status of immune organs, thus protecting broilers from HS-induced injuries. A total of 200 28-day-old Ross 308 broilers were randomly assigned into 5 groups (n = 4 replicates/group, 10 broilers/replicate) which were reared at different ambient temperatures (24 ± 1°C or 33 ± 1°C). The control group fed basal diet, while high-temperature groups were either fed a basal diet (HS group) or a basal diet supplemented with 250-mg Vc/kg diet (HSVc group), 1000-mg Bet/kg diet (HSBet group), and 250-mg Vc plus 1000 mg Bet/kg diet (HSVcBet group), respectively. On day 42, growth performance, humoral immune function, immune organ index, and antioxidant capacity were measured. HS reduced the productive performance of broilers, antibody potency against the Newcastle disease virus (NDV) and sheep red blood cells (SRBC), indices of thymus and bursa, and antioxidant capacity of immune organs. Adding Vc alone or in combination with Bet improved performance, NDV and SRBC antibody potency, thymus and bursa indices, and antioxidant capacity of immune organs in heat-stressed broilers, with the most effective being combination. In summary, HS reduces the antioxidant capacity and immune organ development status of broiler immune organs. Vc and/or Bet can improve the development of immune organs and restore part of the production performance by regulating the antioxidant status of immune organs, among which the combined addition of Vc and Bet has the best effect.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The author would like to thank all teachers and students who dedicated their time to take part in this study.

Funding

The study was funded by the start-up grants to the National Natural Science Foundation of China (No. 31702306); Anhui Province Excellent Young Talents Fund Program of Higher Education Institutions (No. gxyq2020038); Natural science general project of education department of Anhui province (No. KJ2019A0082); and Anhui Provincial Department of Education Outstanding Young Talents Support Project for Universities (No. gxyq2022056).

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Authors and Affiliations

  1. College of Animal Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China

    Shiang Sun, Bing Li, Mingming Wu, Yafei Deng, Jing Li, Yongjie Xiong & Shaojun He

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  1. Shiang Sun
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  2. Bing Li
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  3. Mingming Wu
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  4. Yafei Deng
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  5. Jing Li
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  6. Yongjie Xiong
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  7. Shaojun He
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Contributions

Shiang Sun: conceptualization, methodology, resources, investigation, data curation, analysis, writing—original draft, writing—review and editing, visualization. Bing Li: data curation. Mingning Wu: analysis. Yafei Deng: investigation. Jing Li: methodology, writing—review. Yongjie Xiong: resources, writing—review. Shaojun He: conceptualization, writing—review.

Corresponding author

Correspondence to Shaojun He.

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The ethical approval was obtained from the Institutional Animal Care and Use Committee of Anhui Science and Technology University, China (Approval No. 2022002).

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Sun, S., Li, B., Wu, M. et al. Effect of dietary supplemental vitamin C and betaine on the growth performance, humoral immunity, immune organ index, and antioxidant status of broilers under heat stress. Trop Anim Health Prod 55, 96 (2023). https://doi.org/10.1007/s11250-023-03500-y

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