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Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software

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Abstract

The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.

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Acknowledgements

Authors are grateful to Francisco Buchelly-Imbachi, Juan Ignacio Patore, and Virginia Ballarin (Laboratorio de Procesamiento de Imágenes ICYTE UNMDP–CONICET, Argentina) for providing the software to perform the sperm analysis.

Funding

This research was supported by the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, PICT 2015–3682) and Consejo National de Investigaciones Científicas y Técnicas (CONICET, Argentina, PIP 2014–0273).

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Authors and Affiliations

  1. Biotecnología de La Reproducción, Instituto Nacional de Tecnología Agropecuaria (INTA), Ruta 226 km 73.5, 7620, Balcarce, Argentina

    Alba Ledesma & Federico Hozbor

  2. Consejo Nacional de Investigaciones Cientificas yTécnicas (CONICET), Buenos Aires, Argentina

    Alba Ledesma, Lucía Zalazar, Micaela Greco & Andreina Cesari

  3. Biología de Microorganismos Y Gametas, Instituto de Investigaciones Biológicas CONICET, FCEyN, Universidad Nacional de Mar del Plata, Funes 3250, 7600, Mar del Plata, Argentina

    Lucía Zalazar, Micaela Greco & Andreina Cesari

Authors
  1. Alba Ledesma
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  2. Lucía Zalazar
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  3. Micaela Greco
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  4. Federico Hozbor
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  5. Andreina Cesari
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Contributions

Conception and design of the work: Alba Ledesma, Andreina Cesari. Data collection: Alba Ledesma, Lucía Zalazar, Micaela Greco. Data analysis and interpretation: Alba Ledesma, Lucía Zalazar, Andreina Cesari. Drafting the article: Alba Ledesma. Critical revision of the article: Andreina Cesari, Federico Hozbor.

Corresponding author

Correspondence to Andreina Cesari.

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All procedures involving animals were in accordance with the good animal practice and conditions reviewed and approved by the Animal Ethics Committee of the Instituto Nacional de Tecnología Agropecuaria, Argentina (Protocol ID 156/2018).

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The authors declare no competing interests.

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Ledesma, A., Zalazar, L., Greco, M. et al. Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software. Trop Anim Health Prod 53, 499 (2021). https://doi.org/10.1007/s11250-021-02954-2

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