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Impairment of caprine oocyte maturation in vitro and alteration of granulosa cells functions by widely used fungicide mancozeb

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Abstract

Mancozeb is classified as an endocrine disruptor; thus the present study was carried out to investigate the impact of mancozeb on mammalian ovarian functions using in vitro caprine oocyte maturation and granulosa cell culture models. Caprine cumulus oocyte complexes (COCs) and granulosa cells were cultured under standard culture conditions and treated with mancozeb concentrations of 0.3, 3, and 30 μg/ml along with a control for 24 h and assessed. Granulosa cell viability and progesterone concentration in spent culture media after treatments were also assessed. Mancozeb significantly decreased (P < 0.05) the oocytes cumulus expansion and the maturation of caprine oocytes. Marked changes in granulose cell morphology were observed with 30 μg/ml mancozeb and significantly reduced (P < 0.05) cell viability. Interestingly, the same concentrations significantly increased (P < 0.05) the progesterone secretion by the cells. Significant reduction of granulosa cells viability and reduction of cumulus expansion and suppression of metaphase plate formation in oocyte can impair the fertilization ability and developmental potential of the oocytes. High progesterone concentration due to mancozeb treatment may suppress LH surge and suppress ovulation. In conclusion, mancozeb suppresses granulosa cells viability, reduces cumulus expansion, and suppresses metaphase plate formation but induces progesterone secretion from granulosa cells that may inhibit LH surge for ovulation process.

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The data and other material are not publically available since the project work is still ongoing but can be obtained from the corresponding authors with a reasonable request.

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Acknowledgements

The authors acknowledge the editorial comments made by Dr. Dimanthi V. Jayatilake, Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka.

Funding

This study was partially funded by the grants from the National Science Foundation, Sri Lanka (grant no. NSF /RG/2014/06); International Foundation for Science, Sweden (grant no. B-5367/1); and National Research Council, Sri Lanka (grant no. 13–059) to SPK.

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Authors and Affiliations

  1. Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka

    Ishani Dinisri, Sandarenu Kodikara, Madhusha Prasadani & Suranga Pradeep Kodithuwakku

  2. Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Kamburupitiya, Sri Lanka

    Ishani Dinisri, Sandarenu Kodikara & Indunil Pathirana

  3. Department of Obstetrics & Gynaecology, Faculty of Medicine, University of Peradeniya, Peradeniya, 20400, Sri Lanka

    Chathura Rathnayake

  4. Department of Farm Animal Production & Health, Faculty of Veterinary Medicine & Animal Science, University of Peradeniya, Peradeniya, 20400, Sri Lanka

    Basil Alexander

  5. Department of Obstetrics & Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China

    Kai-Fai Lee & Suranga Pradeep Kodithuwakku

Authors
  1. Ishani Dinisri
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  6. Basil Alexander
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Contributions

SPK, BA, CR, and KFL conceived and designed the research. ID, SK, BA, and MP conducted experiments. IP contributed with the progesterone assays. ID, SK, MP, and SPK analyzed data. ID, MP, KFL, and SPK wrote the manuscript. SPK, CR, and KFL secured funding for research. All authors read and approved the manuscript.

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Dinisri, I., Kodikara, S., Prasadani, M. et al. Impairment of caprine oocyte maturation in vitro and alteration of granulosa cells functions by widely used fungicide mancozeb. Trop Anim Health Prod 53, 406 (2021). https://doi.org/10.1007/s11250-021-02854-5

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