Abstract
Abortion is a major cause of economic loss to the goat industry. Coxiella burnetii the causative agent of Q fever is an important zoonotic agent known to be prevalent worldwide. In the present investigation, we detected the presence of Coxiella burnetii by the modified Ziehl Neelsen method of staining and its DNA by trans-PCR assay in the placenta obtained from the aborted goat. We also ruled out other common causes of abortion in this case. Based on a literature survey, this is the first report on the direct detection of Coxiella burnetii from an aborted goat to be reported from India.
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References
Alton, G.G., Jones, L.M., Piezt, D.E. 1975 Laboratory techniques in Brucellosis. Monogr Ser World Health Organ 55: 1–163.
Ceglie, L., Guerrini, E., Rampazzo, E., Barberio, A., Tilburg, J.J., Hagen, F., Lucchese, L., Zuliani, F., Marangon, S. and Natale, A., 2015. Molecular characterization by MLVA of Coxiella burnetii strains infecting dairy cows and goats of north-eastern Italy, Microbes and Infection, 17, 776–781
Das, D., Malik, S., Mohan, V., Rawool, D. and Barbudhe, S.B., 2013. Screening of fecal droppings of wild birds for coxiellosis by a duplex PCR targeting Com1 and IS1111 genes of Coxiella burnetii, Journal of Foodborne and Zoonotic Diseases, 1, 14–20
Das, D.P., Malik, S., Rawool, D., Das, S., Shoukat, S., Gandham, R.K., Saxena, S., Singh, R., Doijad, S.P. and Barbuddhe, S.B., 2014. Isolation of Coxiella burnetii from bovines with history of reproductive disorders in India and phylogenetic inference based on the partial sequencing of IS1111 element, Infection, Genetics and Evolution, 22, 67–71
Gangoliya, S.R., Kumar, S., Alam, S.I., Devi, D.G. and Guchhait, P., 2016. First molecular evidence of Coxiella burnetii in patients with atypical pneumonia, India, Journal of Medical Microbiology, 65, 255–256
Guatteo, R., Seegers, H., Taurel, A., Joly, A. and Beaudeau, F., 2011. Prevalence of Coxiella burnetii infection in domestic ruminants: A critical review, Veterinary Microbiology,149, 1–16
Kumar, S., Gangoliya, S.R., Alam, S.I., Patil, S., Ajantha, G.S., Kulkarni, R.D. and Kamboj, D.V., 2017. First genetic evidence of Coxiella burnetii in cases presenting with acute febrile illness, India, Journal of Medical Microbiology, 66, 388–390
Malik, S.V.S., Das, D.P., Rawool, D., Kumar, A., Suryawanshi, R.D., Negi, M., Vergis, J., Doijad, S. and Barbuddhe, S.B., 2013. Screening of Foods of Animal Origin for Coxiella burnetii in India, Advances in Animal and Veterinary Sciences, 1, 107–110
Pradeep, J., Stephen, S., Pooja, P., Akshayavardhini, A., Sangeetha, B. and Antony, P.X., 2017. Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants, Veterinary World, 10, 667–671
Rajagunalan, S., Soni, D., Mishra, A., Jeena, L.M., Singh, D.K., Proteen, K., Gunaseelan, L. and Sekar, M., 2016. Molecular Characterization and Typing of Staphylococcus Isolated form nostrils of food handlers, Journal of Cell Tissue Research, 16, 5799–5810.
Roest, H-J., van Gelderen, B., Dinkla, A., Frangoulidis, D., van Zijderveld, F., Rebel, J. and van Keulen, L. 2012. Q Fever in Pregnant Goats: Pathogenesis and Excretion of Coxiella burnetii. PLoS ONE, 7, e48949.
Saini, S., Gupta, V.K., Gururaj, K., Singh, D.D., Pawaiya, R.V.S., Gangwar, N.K., Mishra, A.K., Dwivedi, D., Andani, D., Kumar, A. and Goswami, T.K., 2017. Comparative diagnostic evaluation of OMP31 gene based TaqMan real-time PCR assay with visual LAMP assay and indirect ELISA for caprine brucellosis, Tropical Animal Health and Production, 49, 1253–1264
Sambrook, J. and Russell, D.W., 2006. The condensed protocols from Molecular Cloning: A laboratory Manual. Cold Spring Harbour Laboratory Press, New York.
Schlafer, D.H. and Foster, R.A., 2016. Female Reproductive system, in: Maxie, G.M. (Ed.), Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals, sixth ed. Elsevier, Missorui.
Singh, B. and Prasad, S., 2008. Modelling of Economic Losses due to Some Important Diseases in Goats in India. Agricultural Economics Research Review, 21, 297–302
Smith, M.C. and Sherman, D.M. 2009. Goat Medicine. 2nd edn. Wiley-Blackwell, Ames. pp. 587–588
Stephen, S., Sangeetha, B., Antony, P.X. 2014. Seroprevalence of coxiellosis (Q fever) in sheep & goat in Puducherry and neighbouring Tamil Nadu, Indian Journal of Medical Research, 140, 785
Vaidya, V., Malik, S., Kaur, S., Kumar, S. and Barbuddhe, S., 2008. Comparison of PCR, immunofluorescence assay, and pathogen isolation for diagnosis of Q fever in humans with spontaneous abortions. Journal of Clinical Microbiology, 46, 2038–2044
Vaidya, V.M., Malik, S.V.S., Bhilegaonkar, K.N., Rathore, R.S., Kaur, S. and Barbuddhe, S.B., 2010. Prevalence of Q fever in domestic animals with reproductive disorders, Comparative Immunology, Microbiology and Infectious Diseases, 33, 307–321
Van den Brom, R., van Engelen, E., Roest, H.I., van der Hoek, W. and Vellema, P., 2015. Coxiella burnetii infections in sheep or goats: An opinionated review, Veterinary Microbiology, 181, 119–129
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The authors are thankful to The Dean, VCRI, TANUVAS, Tirunelveli, and The Director, ICAR-CIRG, for providing necessary facilities and funds for carrying out this research work.
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Rajagunalan, S., Gururaj, K., Lakshmikantan, U. et al. Detection of the presence of Coxiella burnetii in a case of goat abortion: a first report from India. Trop Anim Health Prod 51, 983–986 (2019). https://doi.org/10.1007/s11250-018-1756-7
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DOI: https://doi.org/10.1007/s11250-018-1756-7