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Molecular identification of chlamydial cause of abortion in small ruminants in Jordan

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Abstract

Chlamydophila abortus (Ch. abortus) is the etiological agent of ovine enzootic abortion (OEA) and one of the most common infectious agents of abortion in small ruminants worldwide. RFLP-PCR analysis of the outer membrane protein gene (OMP2 gene) was used for diagnosis and characterization of chlamydial causes of abortion in small ruminants in Jordan. Sixty-six placental tissues and 15 vaginal swabs were collected from aborted ewes and does to identify cause of abortion in Jordan. Thirty-eight placental samples (58 %) and 13 vaginal swabs (87 %) were positive for chlamydial DNA. Shedding of bacteria in vaginal swabs was detected within 7 days after abortion. The results of this study showed that chlamydiosis is one of the important causes of abortion in small ruminants in Jordan. In addition, vaginal swab is an excellent sample for molecular diagnosis of chlamydiosis. DNA sequencing and RFLP analysis of the OMP2 reveal that all chlamydial cause of abortion in small ruminants in Jordan are due to Ch. abortus. While, Ch. pecorum was not detected in any sample. OMP2 gene of the isolated Jordanian strain was identical (100 %) to Ch. abortus FAS strain. In conclusion, Ch. abortus is an important cause of abortion in Jordan; vaginal swab within 7 days of abortion can be used for molecular diagnosis of chlamydiosis in small ruminants.

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Acknowledgments

A great acknowledgement is for the Scientific Support Research Fund for financing this project (grant number Z.B/1/10/2010). Also, special thanks for Eng. Faiq Hijazeen, Dr. ebtehal al-Khraisha for their assistance in sample collection and Dr. Amani AL-Dawood for her constructive critique of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. National Center for Agricultural Research and Extension, Baqa’, 19381, Jordan

    Huthaifa Salah Ababneh, Fawzi Mohammad Alsheyab & Moath Ahmad Al-Gharaibeh

  2. Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan

    Mustafa Mohammed Kheir Ababneh, Wael Mahmoud Hananeh & Mohammed Mahmoud Ababneh

  3. Faculty of Agriculture, Jordan University of Science and Technology, Irbid, Jordan

    Khaleel Ibraheem Jawasreh

Authors
  1. Huthaifa Salah Ababneh
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  2. Mustafa Mohammed Kheir Ababneh
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  3. Wael Mahmoud Hananeh
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  4. Fawzi Mohammad Alsheyab
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  5. Khaleel Ibraheem Jawasreh
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  6. Moath Ahmad Al-Gharaibeh
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  7. Mohammed Mahmoud Ababneh
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Correspondence to Huthaifa Salah Ababneh.

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Ababneh, H.S., Ababneh, M.M.K., Hananeh, W.M. et al. Molecular identification of chlamydial cause of abortion in small ruminants in Jordan. Trop Anim Health Prod 46, 1407–1412 (2014). https://doi.org/10.1007/s11250-014-0654-x

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  • DOI: https://doi.org/10.1007/s11250-014-0654-x

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