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Detection and molecular characterization of naturally transmitted sheep associated malignant catarrhal fever in cattle in India

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Abstract

Malignant catarrhal fever (MCF) is a fatal herpesvirus infection of domestic and wild ruminants, with a short and dramatic clinical course characterized primarily by high fever, severe depression, swollen lymph nodes, salivation, diarrhea, dermatitis, neurological disorders, and ocular lesions often leading to blindness. In the present study, fatal clinical cases of sheep associated malignant catarrhal fever (SA-MCF) were identified in cattle in the state of Karnataka. These cases were initially presented with symptoms of diarrhea, respiratory distress, conjunctivitis, and nasal discharges. Laboratory diagnosis confirmed the detection of ovine herpesvirus-2 (OvHV-2) genome in the peripheral blood samples of two ailing animals. The blood samples collected subsequently from sheep of the neighboring areas also showed presence of OvHV-2 genome indicating a nidus of infection in the region. The positive test results were further confirmed by nucleotide sequencing of the OIE approved portion of tegument gene as well as complete ORF8 region of the OvHV-2 genome. Phylogenetic analysis based on the sequence of the latter region indicated close genetic relationship with other OvHV-2 reported elsewhere in the world.

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Acknowledgments

The authors are thankful to the Director IVRI for providing the necessary infrastructure for carrying out the research work. The authors declare no conflict of interest.

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Authors and Affiliations

  1. High Security Animal Disease Laboratory, Anand Nagar, Bhopal, Madhya Pradesh, India

    Richa Sood, Rekha Khandia, Sandeep Bhatia, Manoj Kumar, Atul K. Pateriya, Arshi Siddiqui & Diwakar D. Kulkarni

  2. Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bengaluru, Karnataka, India

    Divakar Hemadri & Sharan S. Patil

  3. Institute of Animal health and Veterinary Biologicals, Southern Regional Disease Diagnostic Laboratory, Hebbal, Bengaluru, Karnataka, India

    Malkanna Sanjeev Kumar & Mudalagiri Dasappa Venkatesha

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  1. Richa Sood
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  2. Rekha Khandia
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Correspondence to Richa Sood.

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Sood, R., Khandia, R., Bhatia, S. et al. Detection and molecular characterization of naturally transmitted sheep associated malignant catarrhal fever in cattle in India. Trop Anim Health Prod 46, 1037–1043 (2014). https://doi.org/10.1007/s11250-014-0611-8

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