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Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing

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Abstract

The synthesis and accumulation of long chain polyunsaturated fatty acids such as eicosapentaenoic acid has previously been demonstrated in the seeds of transgenic plants. However, the obtained levels are relatively low, indicating the need for further studies and the better definition of the interplay between endogenous lipid synthesis and the non-native transgene-encoded activities. In this study we have systematically compared three different transgenic configurations of the biosynthetic pathway for eicosapentaenoic acid, using lipidomic profiling to identify metabolic bottlenecks. We have also used genetic crossing to stack up to ten transgenes in Arabidopsis. These studies indicate several potential approaches to optimize the accumulation of target fatty acids in transgenic plants. Our data show the unexpected channeling of heterologous C20 polyunsaturated fatty acids into minor phospholipid species, and also the apparent negative metabolic regulation of phospholipid-dependent Δ6-desaturases. Collectively, this study confirms the benefits of iterative approaches to metabolic engineering of plant lipid synthesis.

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Acknowledgments

Rothamsted Research receives grant-aided support from BBSRC (UK). The support of BASF Plant Sciences is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biological Chemistry, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK

    Noemi Ruiz-López, Richard P. Haslam, Mónica Venegas-Calerón, Tianbi Li, Johnathan A. Napier & Olga Sayanova

  2. BASF Plant Sciences, Limburgerhof, Germany

    Joerg Bauer

  3. Instituto de la Grasa, CSIC, Av. Padre Garcia Tejero 4, 41012, Seville, Spain

    Mónica Venegas-Calerón

  4. Metanomics GmbH, Tegeler Weg 33, 10589, Berlin, Germany

    Joerg Bauer

Authors
  1. Noemi Ruiz-López
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  2. Richard P. Haslam
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  3. Mónica Venegas-Calerón
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  4. Tianbi Li
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  5. Joerg Bauer
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  6. Johnathan A. Napier
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  7. Olga Sayanova
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Corresponding author

Correspondence to Johnathan A. Napier.

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Ruiz-López, N., Haslam, R.P., Venegas-Calerón, M. et al. Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing. Transgenic Res 21, 1233–1243 (2012). https://doi.org/10.1007/s11248-012-9596-0

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  • DOI: https://doi.org/10.1007/s11248-012-9596-0

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