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Isolation of endophytic bacteria from embryogenic suspension culture of banana and assessment of their plant growth promoting properties

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Abstract

Endophytic microorganisms have been reported from various plants. In the current study, somatic embryogenic cultures of banana (Musa accuminata AAA cv. Grand Naine) were found to have association with endophytic bacteria which were present initially in a covert state. The presence of bacteria was detected only in suspension cultures derived from the somatic embryogenic cultures. The bacteria isolated from embryogenic cell suspension culture were identified as Ralstonia sp. and Bacillus sp. The Ralstonia sp. interestingly showed the presence of various plant growth promoting properties including indole acetic acid and siderophore production. Also the strain was found to have the potential to solubilize phosphate and produce ammonia. Growth enhancement effect of Ralstonia sp. on Vigna radiata seedlings showed promising results and the growth parameters were found to be statistically significant when compared to control. Identification and confirmation of the plant growth promoting properties of Ralstonia sp. makes the study significant with promising applications.

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Abbreviations

IAA:

Indole Acetic Acid

HCN:

Hydrogen Cyanide

ACC:

1-Amino Cyclopropane-1-Carboxylate

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Acknowledgments

Remakanthan A. is grateful to ‘Kerala State Council for Science Technology and Environment’ for a project funded under “Young Investigators Programme in Biotechnology” scheme. Authors also acknowledge the Department of Science and Technology, Government of India support to Mahatma Gandhi University under DST-PURSE program and Department of Biotechnology (DBT), Government of India support to Radhakrishnan E.K. for the project sanctioned under DBT-RGYI scheme for the instrumentation facility.

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Correspondence to E. K. Radhakrishnan.

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Jimtha John C and Smitha PV have contributed equally to this work.

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Jimtha, J.C., Smitha, P.V., Anisha, C. et al. Isolation of endophytic bacteria from embryogenic suspension culture of banana and assessment of their plant growth promoting properties. Plant Cell Tiss Organ Cult 118, 57–66 (2014). https://doi.org/10.1007/s11240-014-0461-0

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