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Agrobacterium-mediated genetic transformation of economically important oilseed rape cultivars

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Abstract

Oilseed rape is considered relatively recalcitrant for genetic modification. This work was performed to establish conditions for effective transformation and regeneration of commercially used cultivars Campino, Haydn, Heros, Hunter and Topas (Brassica napus L.). Cotyledonary petioles and hypocotyls (obtained from the seedlings grown under dark conditions) as a source of explants were used. Our experiments revealed that a lower selection pressure in combination with postponing of selection by 14 days after co-cultivation resulted in recovery of transgenic plants from all cultivars. Transformants were obtained with efficiency from 1 to 5.5%. Histochemically GUS-positive plants were analysed by PCR using primers corresponding to the internal fragments of gus and nptII genes. The transgenic nature was confirmed by Southern blot analyses using a specific nptII probe. This work enriches the list of oilseed rape cultivars available for genetic modification.

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Acknowledgments

We thank Drs. Bodil Jørgensen and Beata Dedicova for their advice concerning oilseed rape transformation. This work was funded by the Slovak Grant Agency VEGA project No. 2-0062-11 and by EEA Financial Mechanism SAV-EHP-2008-02-01.

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Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology, SAS, Akademicka 2, P.O.Box 39A, 95007, Nitra, Slovakia

    Eva Boszoradova, Jana Libantova, Ildiko Matusikova, Zuzana Poloniova, Martin Jopcik & Jana Moravcikova

  2. AIT Austrian Institute of Technology GmbH, Konrad-Lorenz Str. 24, 3430, Tulln, Austria

    Maria Berenyi

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  1. Eva Boszoradova
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  2. Jana Libantova
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  3. Ildiko Matusikova
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  4. Zuzana Poloniova
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  7. Jana Moravcikova
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Correspondence to Jana Moravcikova.

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Boszoradova, E., Libantova, J., Matusikova, I. et al. Agrobacterium-mediated genetic transformation of economically important oilseed rape cultivars. Plant Cell Tiss Organ Cult 107, 317–323 (2011). https://doi.org/10.1007/s11240-011-9982-y

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