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Influence of ectopic expression of Asteraceae MADS box genes on plant ontogeny in tobacco

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Abstract

Plant MADS box transcription factors play key roles in many developmental processes, including the transition to reproductive phase and determination of floral meristem and organs identity. Here we describe the obtaining and characterization of transgenic Nicotiana tabacum L. plants with constitutive expression of Asteraceae MADS box genes CDM111, CDM41, CDM8, CDM77, CDM44 (Chrysanthemum morifolium L.) and HAM92, HAM75 (Helianthus annuus L.). Phylogenetic analysis confirmed that CDM111, HAM75 and HAM92 belong to APETALA1 (AP1), CDM41 and CDM8—FRUITFULL (FUL), CDM44—SEPALLATA3 (SEP3), and CDM77—ASTERACEAE.SEP3 (AST.SEP3) clades. Overexpression of Chrysanthemum and Helianthus AP1/FUL-like genes in tobacco plants resulted in early flowering, shortened stem and decreased number of leaves, which confirmed the functional similarity of Asteraceae AP1/FUL-like factors to AP1 and FUL. This observation testified the conservatism of processes taking place in different plants including Asteraceae. The yeast GAL4 two- and three-hybrid analysis of interactions between CDM77 and other CDM proteins revealed that CDM77 shares similar interaction map with Gerbera SEP-proteins GRCD1 and GRCD2. Overexpression of CDM44 in tobacco caused early flowering without any alterations in vegetative tissues, while overexpression of CDM77 did not reveal any visible developmental changes, which verified the functional similarity between CDM44 and SEP3, and assumed the unique role of CDM77 as whorl- and flower-type specific C-function partner.

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Acknowledgments

The research was supported by the SC No. 02.518.11.7148 and the fundamental investigations program “Molecular and Cell Biology” of Presidium of Russian Academy of Sciences.

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  1. Centre “Bioengineering”, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7, Korp. 1, 117312, Moscow, Russia

    Elena N. Goloveshkina, Anna V. Shchennikova, Anastasia M. Kamionskaya, Konstantin G. Skryabin & Olga A. Shulga

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  1. Elena N. Goloveshkina
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  2. Anna V. Shchennikova
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  3. Anastasia M. Kamionskaya
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Correspondence to Olga A. Shulga.

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Goloveshkina, E.N., Shchennikova, A.V., Kamionskaya, A.M. et al. Influence of ectopic expression of Asteraceae MADS box genes on plant ontogeny in tobacco. Plant Cell Tiss Organ Cult 109, 61–71 (2012). https://doi.org/10.1007/s11240-011-0074-9

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