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Influence of IAA, TDZ, and light quality on asymbiotic germination, protocorm formation, and plantlet development of Cyrtopodium glutiniferum Raddi., a medicinal orchid

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Abstract

We investigated the influence of light quality on in vitro germination and protocorm formation, and the effect of indole-3-acetic acid (IAA) and thidiazuron (TDZ) on proliferation of protocorm-like bodies (PLBs) and development of plantlets of Cyrtopodium glutiniferum Raddi. Germination was faster under white and blue light, and highest under green light. The protocorm developed more rapidly under white, blue, and green light. Continuous darkness delayed seed germination and reduced protocorm formation. Among the plant growth regulators (PGRs) tested for multiplying PLBs, shoots, and roots from protocorms, IAA proved to be superior. TDZ was effective in inducing PLB fresh weight accumulation, but not morphogenesis, unlike IAA. This study indicated that C. glutiniferum seedlings can be produced in vitro using asymbiotic seed germination techniques. High germination rate and protocorm yield can be obtained by initially cultivating C. glutiniferum seeds on medium without growth regulators under white light, or under white light supplemented with green or blue light. This culture system complies with commercial and conservation requirements for rapid and low-cost propagation.

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Abbreviations

ANOVA:

Analysis of variance

BL:

Blue light

D:

Darkness

GL:

Green light

IAA:

Indole-3-acetic acid

MS0:

Liquid culture medium without growth regulators (Murashige and Skoog 1962)

NAA:

1-naphthaleneacetic acid

PGRs:

Plant growth regulators

PLBs:

Protocorm-like bodies

RL:

Red light

SE:

Standard error

TDZ:

Thidiazuron

UV:

Ultraviolet light

WL:

White light

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Acknowledgments

The authors thank the Estácio de Sá University (Pharmacy Course, Unidade Rebouças) for providing fellowships and financial support for this project, and the Federal University of the State of Rio de Janeiro (UNIRIO) for providing the necessary facilities. Drs. Frederico Augusto Furtado and Janet W. Reid revised the English text.

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Authors and Affiliations

  1. Laboratório de Cultura de Células, Tecidos Vegetais e Investigação Fitoquímica, Curso de Farmácia, Universidade Estácio de Sá, Rio de Janeiro, RJ, Brazil

    Ingrid N. Vogel

  2. Laboratório Integrado de Biologia Vegetal, Departamento de Botânica, Instituto de Biociências, CCBS, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Andrea F. Macedo

  3. Avenida Pasteur, 458, 5° andar, sala 512C, Urca, Rio de Janeiro, CEP 22.290.240, RJ, Brazil

    Andrea F. Macedo

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  1. Ingrid N. Vogel
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Correspondence to Andrea F. Macedo.

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Vogel, I.N., Macedo, A.F. Influence of IAA, TDZ, and light quality on asymbiotic germination, protocorm formation, and plantlet development of Cyrtopodium glutiniferum Raddi., a medicinal orchid. Plant Cell Tiss Organ Cult 104, 147–155 (2011). https://doi.org/10.1007/s11240-010-9810-9

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