Abstract
We conducted a systematic assessment and comparative study on the biochemical and cellular characteristics of cultured cotton cells during the entire process of somatic embryogenesis (SE). All staged cultures were widely investigated in this assay. Cell and tissue ectogenesis manipulation combined with flow cytometry (FCM) was employed to cellular study during the whole totipotency process of dedifferentiation and redifferentiation. We identified two phases of chromatin decondensation during the dedifferentiation and redifferentiation. At the same time, sharp increase in the ratio of indoleacetic acid (IAA), isopentenyladenosine group (iPAs) at the same stage of cell dedifferentiation and redifferentiation process serve as distinct biochemical maker of dedifferentiation and SE initiation with the unique feature. Our results suggest the two phases of chromatin reorganization associated with endogenous auxin/cytokinin dynamic activity may underlie dedifferentiation and redifferentiation during the entire SE process in cotton.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- EC:
-
Embryogenic calli
- FCM:
-
Flow cytometry
- MS:
-
Murashige and Skoog medium
- NEC:
-
Nonembryogenic calli
- PEM:
-
Proembryogenic masses
- SE:
-
Somatic embryogenesis
- IAA:
-
Indoleacetic acid
- iPAs:
-
Isopentenyladenosine group
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Acknowledgements
We thank Yupeng Fan and Dingli Li for excellent technical assistance. This work was supported by a Program for New Century Excellent Talents in University, Ministry of Education of China.
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Zeng, F., Zhang, X., Jin, S. et al. Chromatin reorganization and endogenous auxin/cytokinin dynamic activity during somatic embryogenesis of cultured cotton cell. Plant Cell Tiss Organ Cult 90, 63–70 (2007). https://doi.org/10.1007/s11240-007-9253-0
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DOI: https://doi.org/10.1007/s11240-007-9253-0