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Augmentin® as an alternative antibiotic for growth suppression of Agrobacterium for tomato (Lycopersicon esculentum) transformation

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Abstract

Augmentin® was used as an alternative antibiotic for suppressing Agrobacterium tumefaciens during tomato (Lycopersicon esculentum) transformation. The efficiency of Augmentin compared with Timentin® to suppress the growth of Agrobacterium was at concentrations of 300 and 100 mg l−1, respectively. In addition, the high concentration up to 500 mg l−1 of both antibiotics showed no significant toxicity to shoot regeneration. The overgrowth of Agrobacterium was observed on tomato shoots regenerated on medium containing cefotaxime even at high concentration up to 500 mg l−1. Moreover, shoot regeneration from medium containing cefotaxime was lower than the one from medium containing Timentin and Augmentin. However, we found that Timentin was more stable than Augmentin when stored as a mixed stock solution and kept at −20°C freezer. In order to increase the efficiency of Augmentin, we recommended the use of Augmentin at a concentration of 300 mg l−1 with fresh preparation prior to use. Therefore, Augmentin can be used as another antibiotic for suppression of bacterial growth with comparative efficiency as Timetin for tomato transformation.

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Abbreviations

MS medium:

Murashige and Skoog medium

MS-B5 medium:

MS medium containing B5 vitamins

References

  • MK Alsheikh HP Suso M Robson NH. Battey A Wetten (2002) ArticleTitleAppropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens Plant Cell Rep. 20 1173–1180 Occurrence Handle1:CAS:528:DC%2BD38Xks12qsbs%3D

    CAS  Google Scholar 

  • SR. Bhat S Srinivisan (2002) ArticleTitleMolecular and genetic analyses of transgenic plants: consideration and approaches Plant Sci. 163 673–681 Occurrence Handle1:CAS:528:DC%2BD38XnsVShu7g%3D

    CAS  Google Scholar 

  • M Chabaud F. Carvalho-Niebel Particlede DG Barker (2003) ArticleTitleEfficient transformation of Medicago truncata cv Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL 1 Plant Cell Rep. 22 46–51 Occurrence Handle1:CAS:528:DC%2BD3sXmtFCjsLc%3D Occurrence Handle12827434

    CAS  PubMed  Google Scholar 

  • ZM Cheng JA. Schnurr JA Kapaun (1998) ArticleTitleTimentin as an alternative antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation Plant Cell Rep. 17 646–649 Occurrence Handle1:CAS:528:DyaK1cXivFylu78%3D

    CAS  Google Scholar 

  • MGC Costa FTS Nogueira ML Figueira WC Otoni SH. Brommonschenkel PR Cecon (2000) ArticleTitleInfluence of the antibiotic timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars Plant Cell Rep. 19 327–332 Occurrence Handle1:CAS:528:DC%2BD3cXjsVeitbg%3D

    CAS  Google Scholar 

  • S Dronne S Moja F Jullien F. Berger FC Caissard (1999) ArticleTitleAgrobacterium-mediated transformation of lavandin (Lavandula × intermedia Emeric ex Loiseleur) Transgenic Res. 8 335–347 Occurrence Handle1:CAS:528:DC%2BD3cXosFGltA%3D%3D

    CAS  Google Scholar 

  • M Estopà V Marfà E. Mele′ J Messeguer (2001) ArticleTitleStudy of different antibiotic combinations for use in the elimination of Agrobacterium with kanamycin selection in carnation Plant Cell Tiss. Org. Cult. 65 211–220

    Google Scholar 

  • TM Fluton J. Chunwongse SD Tanksley (1995) ArticleTitleMiniprep protocol for extraction of DNA from tomato and herbaceous plant Plant Mol. Rep. 13 IssueID3 207–209

    Google Scholar 

  • OL Gamborg RA. Miller K Ojima (1968) ArticleTitleNutrient requirement of suppression cultures of soybean root cells Exp. Cell. Res. 50 151–158 Occurrence Handle1:CAS:528:DyaF1cXktVyqtLw%3D Occurrence Handle5650857

    CAS  PubMed  Google Scholar 

  • P. Holford HJ Newbury (1992) ArticleTitleThe effects of antibiotics and their breakdown products on the in vitro growth of Antirrhinum majus Plant Cell Rep. 11 93–96 Occurrence Handle1:CAS:528:DyaK38XktVCgtrw%3D

    CAS  Google Scholar 

  • RS Jiang JW. Jang CY Hsu (2001) ArticleTitleBacteriology of chronic sinusitis after amoxicillin–clavulanate potassium therapy Arch. Otolaryngol. Head Neck Surg. 124 683–686 Occurrence Handle1:STN:280:DC%2BD3MzitVGntg%3D%3D

    CAS  Google Scholar 

  • JJ Lin N. Assad-Garcia J Kuo (1995) ArticleTitlePlant hormone effect of antibiotics on the transformation efficiency of plant tissue by Agrobacterium tumefaciens cells Plant Sci. 109 171–177 Occurrence Handle1:CAS:528:DyaK2MXntl2ksL8%3D

    CAS  Google Scholar 

  • HQ Ling D. Kriseleit MW Ganal (1998) ArticleTitleEffect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.). Plant Cell Rep 17 843–847 Occurrence Handle1:CAS:528:DyaK1cXltFKls7o%3D

    CAS  Google Scholar 

  • Maneekard K (2003) Study of factors affecting on efficiency of gene transfer into tomato using mini-binary vector. MS Thesis, Kasetsart University, Bangkok, Thialand (p. 106)

  • Maneekard K, Rodaree R., Chatchawankanphanich O (2003) Effect of antibiotic cefotaxime and timentin on regeneration of tomato after transformation. The Proceedings of 41th Kasetsart University Annual Conference, Kasetsart University, Bangkok, Thailand (pp. 318–325)

  • RJ. Mathias LA Boyd (1986) ArticleTitleCefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L EM. THELL). Plant Sci. 46 217–223 Occurrence Handle1:CAS:528:DyaL2sXjtVWnsA%3D%3D

    CAS  Google Scholar 

  • LA Miller K. Ratnam DJ Payne (2001) ArticleTitleβ-lactamase-inhibitor combinations in the 21st century: current agents and new developments Curr. Opin. Pharmacol. 1 451–458 Occurrence Handle1:CAS:528:DC%2BD3MXnsFOjur8%3D Occurrence Handle11764769

    CAS  PubMed  Google Scholar 

  • T. Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant. 15 473–497 Occurrence Handle1:CAS:528:DyaF3sXksFKm

    CAS  Google Scholar 

  • B Nauerby K. Billing R Wyndaele (1997) ArticleTitleInfluence of the antibiotic timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens Plant Sci. 123 169–177 Occurrence Handle1:CAS:528:DyaK2sXitVynsL4%3D

    CAS  Google Scholar 

  • H Tang Z. Ren G Krczal (2000) ArticleTitleAn evaluation of antibiotics for the elimination of Agrobacterium tumefaciens from walnut somatic embryos and for the effects on the proliferation of somatic embryos and regeneration of transgenic plants Plant Cell Rep. 19 881–887 Occurrence Handle1:CAS:528:DC%2BD3cXmt12isrg%3D

    CAS  Google Scholar 

  • A Vergauwe R Cammaert D Vandenberghe C Genetello D Inzé M. Van Montagu E Vanden Eeckhout (1996a) ArticleTitleAgrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants Plant Cell Rep. 15 929–933 Occurrence Handle1:CAS:528:DyaK28Xmt1artL8%3D

    CAS  Google Scholar 

  • A Vergauwe E Van Geldre D Inzé M. Van Montagu E Van den Eeckhout (1996b) ArticleTitleThe use of amoxicillin and ticarcillin in combination with a β-lactamase inhibitor as decontaminating agents in the Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. J. Biotech. 52 89–95 Occurrence Handle1:CAS:528:DyaK2sXptlWjtA%3D%3D

    CAS  Google Scholar 

  • AR White C Kaye J Poupard R Pypstra G. Woodnutt B Wynne (2004) ArticleTitleAugmentin® (amoxicillin/clavulanate) in the treatment of community-acquired respiratory tract infection: a review of the continuing development of an innovative antimicrobial agent J. Antimicrob. Chemother 53 IssueIDSuppl S1 i3–i20 Occurrence Handle1:CAS:528:DC%2BD2cXitFKitb8%3D Occurrence Handle14726431

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen campus, Nakhon Pathom, 73140, Thailand

    S. Ieamkhang

  2. Plant Genetic Engineering Unit (PGEU), National Center for Genetic Engineering and Biotechnology (BIOTEC), Kasetsart University, Kamphaengsaen campus, Nakhon Pathom, 73140, Thailand

    O. Chatchawankanphanich

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  1. S. Ieamkhang
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  2. O. Chatchawankanphanich
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Correspondence to O. Chatchawankanphanich.

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Ieamkhang, S., Chatchawankanphanich, O. Augmentin® as an alternative antibiotic for growth suppression of Agrobacterium for tomato (Lycopersicon esculentum) transformation. Plant Cell Tiss Organ Cult 82, 213–220 (2005). https://doi.org/10.1007/s11240-005-0416-6

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