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Molecular phylogeny of two Rhinoxenus species (Monogenea: Dactylogyridae) from the nasal cavities of serrasalmids (Characiformes: Serrasalmidae) from Brazil

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Abstract

The dactylogyrid genus Rhinoxenus (Monogenea: Dactylogyridae) is composed of specialized monogeneans infecting the nasal cavities of freshwater fishes from the Neotropical region. This taxon currently comprises 11 species and is easily distinguished from other monogeneans by the absence of the dorsal bar, ventral anchor with inconspicuous roots covered by a sclerotized cap, dorsal anchor greatly modified into a needle-like shape, and hook pair 2 located into bilateral lobes of the trunk. Specimens of Rhinoxenus euryxenus and Rhinoxenus paranaensis were found infecting the nasal cavities of Serrasalmus marginatus and Serrasalmus maculatus, respectively, from the Paraná River basin in Brazil. For the first time, molecular data of Rhinoxenus spp. were obtained and used as a basis for phylogenetic analyses of the genus. Furthermore, our findings represent the first record of R. paranaensis in Brazil.

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All data will be available for consultation. Whenever necessary, they should be requested from the corresponding author. The specimens used for this work will be available in specialized scientific collections.

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Funding

We thank the agencies for the financial support to this study: FAPESP – São Paulo Research Foundation [grant number – #2020/05412-9; D.H.M.D.V.: 2019/19060-0; R.B.N.: 2019/26831-2; M.B.E.: 2021/12779-9; M.I.M.: 2017/16546-3], and CNPq - National Council for Scientific and Technological Development [grant number – M.M.O.P.: 161838/2021-9; R.J.S.: 311635/2021-0]; M.B.E. was also supported by the Pro-Rectory of Research (PROPG/PROPe – UNESP, Edital 04/2022].

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Authors and Affiliations

  1. Institute of Biosciences, Division of Parasitology, São Paulo State University (UNESP), Botucatu, São Paulo, 18618-689, Brazil

    Melissa Miyuki Osaki-Pereira, Rodrigo Bravin Narciso, Diego Henrique Mirandola Dias Vieira, Mariana Bertholdi Ebert & Reinaldo José da Silva

  2. Department of Biological Sciences, Federal University of São Paulo (UNIFESP), Rua Professor Arthur Riedel, 275, Jardim Eldorado, Diadema, São Paulo, 09972-270, Brazil

    Maria Isabel Müller

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  1. Melissa Miyuki Osaki-Pereira
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Contributions

All authors contributed to the study's conception and design. Material preparation, data collection, and analysis were performed by M.M.O.P, R.B.N, D.H.M.D.V, M.I.M, M.B.E, and R.J.S. The first version of the manuscript was written by M.M.O.P and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Melissa Miyuki Osaki-Pereira.

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11230_2023_10102_MOESM1_ESM.docx

Supplementary file1 (DOCX 31 KB) Supplementary information 1. List of monogeneans included in the phylogenetic analyses, with details of the host, locality, and GenBank accession numbers of sequences from the partial 28S rDNA. New sequences obtained for the present study are in bold.

11230_2023_10102_MOESM2_ESM.docx

Supplementary file2 (DOCX 75 KB) Supplementary information 2. Genetic divergence (expressed in %) estimated for partial sequences of the 28S rDNA (LSU) gene within species of Dactylogyridae retrieved from GenBank.

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Osaki-Pereira, M.M., Narciso, R.B., Vieira, D.H.M.D. et al. Molecular phylogeny of two Rhinoxenus species (Monogenea: Dactylogyridae) from the nasal cavities of serrasalmids (Characiformes: Serrasalmidae) from Brazil. Syst Parasitol 100, 521–530 (2023). https://doi.org/10.1007/s11230-023-10102-7

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