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Description of two new species of Myxobolus Bütschli, 1892, M. peleci n. sp. and M. cultrati n. sp., detected during an intensive mortality of the sichel, Pelecus cultratus (L.) (Cyprinidae), in Lake Balaton, Hungary

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Abstract

In the summer of 2014, mass mortality of the sichel, Pelecus cultratus (L.), was observed in Lake Balaton, Hungary. Parasitological examination conducted in the framework of a complete diagnostic survey revealed myxozoan infections. Two species of Myxobolus Bütschli, 1892 were found, one in the gill lamellae and another in the eyes. Following this mass mortality, 113 sichel specimens were examined during a 14-month period. Gill infection with scattered spores in the lamellae was found in 51 fish, while infection in the eyes was recorded in three specimens only. Based upon the morphological and molecular biological data the species from the gills is described here as Myxobolus peleci n. sp. and the species from the eye as M. cultrati n. sp. The 18S rDNA sequences of the two species proved that they differ from all known Myxobolus spp. with sequence data available in the GenBank database. Histological examinations revealed that the spores found in the gill lamellae were derived from plasmodia developing in and around the afferent branchial arteries of the gill arches. No mortality of sichel was recorded in 2015. Infection with these two Myxobolus spp. does not seem to play a role in the mortality of the host fish.

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Acknowledgements

The authors thank the Balaton Fishery Ltd. and Drs András Specziár and Tibor Erős (senior scientists at the Balaton Limnological Institute, Centre for Ecological Research, Hungarian Academy of Sciences), for helping in the collection of sichel, and Ms. Györgyi Pataki for preparing the drawings and histological slides.

Funding

The study was supported by the Hungarian Scientific Research Fund (OTKA, project no. K100132).

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Authors and Affiliations

  1. Centre for Agricultural Research, Institute for Veterinary Medical Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest, 1143, Hungary

    Réka Borzák, Kálmán Molnár, Gábor Cech & Csaba Székely

  2. National Food Chain Safety Office – Veterinary Diagnostic Directorate, Laboratory for Fish and Bee Diseases, Tábornok u. 2, Budapest, 1143, Hungary

    Melitta Papp & Petra Deák-Paulus

Authors
  1. Réka Borzák
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  2. Kálmán Molnár
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  3. Gábor Cech
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  5. Petra Deák-Paulus
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  6. Csaba Székely
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Correspondence to Réka Borzák.

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Ethical approval

All applicable institutional, national and international guidelines for the care and use of animals were followed. Fishing was carried out with fishing permit for research purposes: HHgF/110-2/2016, Ministry of Agriculture, Hungary.

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Borzák, R., Molnár, K., Cech, G. et al. Description of two new species of Myxobolus Bütschli, 1892, M. peleci n. sp. and M. cultrati n. sp., detected during an intensive mortality of the sichel, Pelecus cultratus (L.) (Cyprinidae), in Lake Balaton, Hungary. Syst Parasitol 93, 667–677 (2016). https://doi.org/10.1007/s11230-016-9651-y

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