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Modification of dipeptides by alkyl chloroformate—alkanol mixtures for analysis by gas chromatography/mass spectrometry with electron and chemical ionization and collisional activation: Differentiation of isomers

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Abstract

A possibility of dipeptide derivatization by alkyl chloroformate—alkanol (Alk = Me, Et, Pr, Bu) mixed reactants for their determination and differentiation of isomers in mixtures by gas chromatography/mass spectrometry with electron and chemical ionization and collisional activation was studied. Prospects for using derivatization by mixtures of methyl chloroformate with 2,2,2-trifluoroethanol, 2,2,3,3,3-pentafluoropropanol, and 2,2,3,3,4,4,4-heptafluorobutanol were shown. Diagnostic ions that make it possible to determine the amino acid sequence in dipeptides and distinguish residues of isomeric leucine and isoleucine were revealed.

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Authors and Affiliations

  1. A. V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, 29 Leninsky prosp., 119991, Moscow, Russian Federation

    R. S. Borisov & V. G. Zaikin

  2. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, 117997, Moscow, Russian Federation

    B. V. Vas’kovskii

  3. Institute of Immunology, 24-4 Kashirskoe sh., 115478, Moscow, Russian Federation

    L. Yu. Sklyarov

Authors
  1. R. S. Borisov
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  2. V. G. Zaikin
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  3. B. V. Vas’kovskii
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  4. L. Yu. Sklyarov
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Corresponding author

Correspondence to V. G. Zaikin.

Additional information

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 12, pp. 2200–2204, December, 2006.

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Borisov, R.S., Zaikin, V.G., Vas’kovskii, B.V. et al. Modification of dipeptides by alkyl chloroformate—alkanol mixtures for analysis by gas chromatography/mass spectrometry with electron and chemical ionization and collisional activation: Differentiation of isomers. Russ Chem Bull 55, 2285–2290 (2006). https://doi.org/10.1007/s11172-006-0584-5

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  • DOI: https://doi.org/10.1007/s11172-006-0584-5

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