Abstract
Some fundamental aspects of the immobilization of a hydrolytic enzyme glucoamylase via adsorption on aminocarboxylic ion-exchangers with fibrous structure were considered. The sorption capacity of amphoteric supports for the enzyme was studied in relation to the time of immobilization, concentration of hydrogen ions and the protein, and the ionic form of a polyelectrolyte. The catalytic properties of free and immobilized enzymes were compared.
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REFERENCES
Zherebtsov, N.A., Amiloliticheskie fermenty v pishchevoi promyshlennosti (Amylolytic Enzymes in Food Industry), Moscow: Leg. Pishch. Prom-st., 1984.
Halgas, J., Biocatalysts in Organic Synthesis, Amsterdam: Elsevier, 1992.
Kovalenko, G.A., Komova, V.A., Simakov, A.V., et al., Biotekhnologiya, 2002, no. 3, pp. 55–66.
Microbial Enzymes and Biotechnology, Fogarty, W.M., Ed., Elsevier Applied Scince, 1983. Translated under the title Mikrobnye fermenty i biotekhnologiya, Moscow: Agropromizdat, 1986.
RF Patent 2 181 770.
Shkutina, I.V., Stoyanova, O.F., and Selemenev, V.F., Zh. Prikl. Khim., 2001, vol. 74, no.5, pp. 869–871.
Shkutina, I.V., Stoyanova, O.F., Selemenev, V.F., and Grigor’eva, G.A., Zh. Fiz. Khim., 2001, vol. 75, no.11, pp. 2008–2010.
Soldatov, V.S., Martsinkevich, R.V., Pokrovskaya, A.I., and Shunkevich, A.A., Zh. Prikl. Khim., 1994, vol. 67, no.10, pp. 1640–1643.
Soldatov, V.S., Martsinkevich, R.V., Pokrovskaya, A.I., and Shunkevich, A.A., Zh. Prikl. Khim., 1994, vol. 67, no.10, pp. 1644–1647.
Selemenev, V.F., Slavinskaya, G.V., Khokhlov, V.Yu., et al., Praktikum po ionnomu obmenu (Laboratory Manual of Ion Exchange), Voronezh: Voronezh. Gos. Univ., 2004.
Lowry, O.H., J. Biol. Chem., 1951, vol. 193, pp. 265–275.
Chibata, I., Pure Appl. Chem., 1978, vol. 50, no.7, pp. 667–675.
Shataeva, L.K., Kuznetsova, N.N., and El’kin, G.E., Karboksil’nye kationity v biotekhnologii (Carboxylic Cationites in Biotechnology), Leningrad: Nauka, 1979.
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Translated from Zhurnal Prikladnoi Khimii, Vol. 78, No. 6, 2005, pp. 1003–1005.
Original Russian Text Copyright © 2005 by Shkutina, Stoyanova, Selemenev.
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Shkutina, I.V., Stoyanova, O.F. & Selemenev, V.F. Specific Features of Glucoamylase Immobilization by Adsorption on Fibrous Polyelectrolytes. Russ J Appl Chem 78, 981–984 (2005). https://doi.org/10.1007/s11167-005-0432-7
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DOI: https://doi.org/10.1007/s11167-005-0432-7