Skip to main content
Log in

Specific Features of Glucoamylase Immobilization by Adsorption on Fibrous Polyelectrolytes

  • Macromolecular Chemistry and Polymeric Materials
  • Published:
Russian Journal of Applied Chemistry Aims and scope Submit manuscript

Abstract

Some fundamental aspects of the immobilization of a hydrolytic enzyme glucoamylase via adsorption on aminocarboxylic ion-exchangers with fibrous structure were considered. The sorption capacity of amphoteric supports for the enzyme was studied in relation to the time of immobilization, concentration of hydrogen ions and the protein, and the ionic form of a polyelectrolyte. The catalytic properties of free and immobilized enzymes were compared.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Zherebtsov, N.A., Amiloliticheskie fermenty v pishchevoi promyshlennosti (Amylolytic Enzymes in Food Industry), Moscow: Leg. Pishch. Prom-st., 1984.

    Google Scholar 

  2. Halgas, J., Biocatalysts in Organic Synthesis, Amsterdam: Elsevier, 1992.

    Google Scholar 

  3. Kovalenko, G.A., Komova, V.A., Simakov, A.V., et al., Biotekhnologiya, 2002, no. 3, pp. 55–66.

  4. Microbial Enzymes and Biotechnology, Fogarty, W.M., Ed., Elsevier Applied Scince, 1983. Translated under the title Mikrobnye fermenty i biotekhnologiya, Moscow: Agropromizdat, 1986.

  5. RF Patent 2 181 770.

  6. Shkutina, I.V., Stoyanova, O.F., and Selemenev, V.F., Zh. Prikl. Khim., 2001, vol. 74, no.5, pp. 869–871.

    Google Scholar 

  7. Shkutina, I.V., Stoyanova, O.F., Selemenev, V.F., and Grigor’eva, G.A., Zh. Fiz. Khim., 2001, vol. 75, no.11, pp. 2008–2010.

    Google Scholar 

  8. Soldatov, V.S., Martsinkevich, R.V., Pokrovskaya, A.I., and Shunkevich, A.A., Zh. Prikl. Khim., 1994, vol. 67, no.10, pp. 1640–1643.

    Google Scholar 

  9. Soldatov, V.S., Martsinkevich, R.V., Pokrovskaya, A.I., and Shunkevich, A.A., Zh. Prikl. Khim., 1994, vol. 67, no.10, pp. 1644–1647.

    Google Scholar 

  10. Selemenev, V.F., Slavinskaya, G.V., Khokhlov, V.Yu., et al., Praktikum po ionnomu obmenu (Laboratory Manual of Ion Exchange), Voronezh: Voronezh. Gos. Univ., 2004.

    Google Scholar 

  11. Lowry, O.H., J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    PubMed  Google Scholar 

  12. Chibata, I., Pure Appl. Chem., 1978, vol. 50, no.7, pp. 667–675.

    Google Scholar 

  13. Shataeva, L.K., Kuznetsova, N.N., and El’kin, G.E., Karboksil’nye kationity v biotekhnologii (Carboxylic Cationites in Biotechnology), Leningrad: Nauka, 1979.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

__________

Translated from Zhurnal Prikladnoi Khimii, Vol. 78, No. 6, 2005, pp. 1003–1005.

Original Russian Text Copyright © 2005 by Shkutina, Stoyanova, Selemenev.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shkutina, I.V., Stoyanova, O.F. & Selemenev, V.F. Specific Features of Glucoamylase Immobilization by Adsorption on Fibrous Polyelectrolytes. Russ J Appl Chem 78, 981–984 (2005). https://doi.org/10.1007/s11167-005-0432-7

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11167-005-0432-7

Keywords

Navigation