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Hibiscus Acid Inhibitory Capacity of Angiotensin Converting Enzyme: an In Vitro and In Silico Study

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Data Availability

All data generated or analyzed during this study are included in this publication article (and its Supplementary information files).

References

  1. Al Ghorani H, Kulenthiran S, Lauder L, Böhm M, Mahfound F, Al Ghorani H (2021) Hypertension trials update. J Hum Hypertens 35(5):398–409. https://doi.org/10.1038/s41371-020-00477-1

    Article  PubMed  PubMed Central  Google Scholar 

  2. Al-Makki A, DiPette D, Whelton P, Murad H, Mustafa R, Acharya S et al (2022) Hypertension pharmacological treatment in adults: a world health organization guideline executive summary. Hypertension 79:293–301. https://doi.org/10.1161/HYPERTENSIONAHA.121.18192

    Article  CAS  Google Scholar 

  3. Ram C (2002) Antihypertensive drugs: an overview. Am J Cardiovasc Drugs 2:77–89. https://doi.org/10.2165/00129784-200202020-00002

    Article  CAS  PubMed  Google Scholar 

  4. Laurent S (2017) Antihypertensive drugs. Pharmacol Res 124:116–125. https://doi.org/10.1016/j.phrs.2017.07.026

    Article  CAS  PubMed  Google Scholar 

  5. Bragueto-Escher G, Boscacci-Marques M, Araújo-Vieira do Carmo M, Azevedo L, Miranda-Furtado M, Sant´Ana A et al (2019) Clitoria ternatea petal bioactive compounds display antioxidant, antihemolytic and antihipertensive effects, inhibit a-amylase and a-glucosidase activities and reduce human LDL cholesterol and DNA induced oxidation. Food Res Int 128:108763. https://doi.org/10.1016/j.foodres.2019.108763

    Article  CAS  Google Scholar 

  6. Cú-Cañetas T, Betancur-Ancona D, Gallegos-Tintoré S, Sandoval-Peraza M, Chel-Guerrero L (2015) Estudios de inhibición in vitro de la enzima convertidora de angiotensina-I, efectos hipotensor y antihipertensivo de fracciones peptídicas de V. Unguiculata. Nutri Hosp 35:2117–2125. https://doi.org/10.3305/nh.2015.32.5.9624

    Article  CAS  Google Scholar 

  7. Arroyo J, Raez E, Rodríguez M, Chumpitaz V, Burga J, De la Cruz W, Valencia  J (2008) Actividad antihipertensiva y antioxidante del extracto hidroalcohólico atomizado de Maíz morado (Zea mays L) en ratas. Revista Peruana de medicina experimental y Salud Pública 25(2):195–199

  8. Actis-Goretta L, Ottavianni J, Fraga C (2006) Inhibition of angiotensin converting enzyme activity by flavanol-rich foods. J Agric Food Chem 54:229–234. https://doi.org/10.1021/jf052263o

    Article  CAS  PubMed  Google Scholar 

  9. Ojeda D, Jiménez-Ferrer E, Zamilpa A, Herrera-Arellano A, Tortoriello J, Alvarez L (2010) Inhibition of angiotensin converting enzyme (ACE) activity by the anthocyanins delphinidin and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa. J Ethnopharmacol 127:7–10. https://doi.org/10.1016/j.jep.2009.09.059

    Article  CAS  PubMed  Google Scholar 

  10. Parichatikanond W, Pinthong D, Mangmool S (2012) Blockade of the renin-angiotensin system with delphinidin, cyanin and quercetin. Planta Med 78:1626–1632. https://doi.org/10.1055/s-0032-1315198

    Article  CAS  PubMed  Google Scholar 

  11. Alarcón-Alonso J, Zamilpa A, Alarcón-Aguilar F, Herrera-Ruiz M, Tortoriello J, Jimenez-Ferrer E (2012) Pharmacological characterization of the diuretic effect of Hibiscus sabdariffa Linn (Malvaceae) extract. J Ethnopharmacol 139:751–756. https://doi.org/10.1016/j.jep.2011.12.005

    Article  CAS  PubMed  Google Scholar 

  12. Vargas-León E, Díaz-Batalla L, González-Cruz L, Bernardino-Nicanor A, Castro-Rosas J, Reynoso-Camacho R, Gómez-Aldapa C (2018) Effects of acid hydrolysis on the free radical scavenging capacity and inhibitory activity of the angiotensin converting enzyme of phenolic compounds of two varieties of two varieties of Jamaica (Hibiscus sabdariffa). Ind Crops Prod 116:201–208. https://doi.org/10.1016/j.indcrop.2018.02.044

    Article  CAS  Google Scholar 

  13. Salem M, Michel H, Ezzat M, Okba M, EL-Desoky A, Mohamed S, Ezzat S (2020) Optimization of an extraction solvent for angiotensin-converting enzyme inhibitors from Hibiscus sabdariffa L. based on its UPLC-MS/MS metabolic profiling. Mol 25:2307. https://doi.org/10.3390/molecules25102307

    Article  CAS  Google Scholar 

  14. Agarwal S, Mehrotra R (2016) An overview of molecular docking. JSM Chem 4:1024–1028

    Google Scholar 

  15. Hassan ST, Švajdlenka E (2017) Biological evaluation and molecular docking of protocatechuic acid from Hibiscus sabdariffa L. as a potent urease inhibitor by an ESI-MS based method. Mol 22:1696. https://doi.org/10.3390/molecules22101696

    Article  CAS  Google Scholar 

  16. Giacoman-Martínez A, Alarcón-Aguilar F, Zamilpa A, Hidalgo-Figueroa S, Navarrete-Vázquez G, García-Macedo R et al (2019) Triterpenoids from Hibiscus sabdariffa L. with PPARδ/γ dual agonist action: in vivo, in vitro and in silico studies. Planta Med 85:412–423. https://doi.org/10.1055/a-0824-1316

    Article  CAS  PubMed  Google Scholar 

  17. Ajay M, Chai H, Mustafa A, Gilani A, Mustafa M (2007) Mechanisms of the anti-hypertensive effect of Hibiscus sabdariffa L. calyces. J Ethnopharmacol 109:388–393. https://doi.org/10.1016/j.jep.2006.08.005

    Article  CAS  PubMed  Google Scholar 

  18. García-Álvarez R, Ramírez-Mendiola B, Coria-Jiménez R, Ortíz-Herrera M, Chavez-Pacheco J, Alemón-Medina R (2014) Estabilidad fisicoquímica y microbiológica de captopril en formulación extemporánea. Acta Pediatr Méx 35:459–468

    Google Scholar 

  19. Heran B, Wong M, Heran I, Wright J (2008) Blood pressure lowering efficacy of angiotensin converting enzyme (ACE) inhibitors for primary hypertension. Cochrane Database Syst Rev 4:CD003823. https://doi.org/10.1002/14651858.CD003823.pub2

    Article  Google Scholar 

  20. Hopkins A, Lamm M, Funk J, Ritenbaugh C (2013) Hibiscus sabdariffa L. in the treatment of hypertension and hyperlipidemia: a comprehensive review of animal and human studies. Fitoterapia 85:84–94. https://doi.org/10.1016/j.fitote.2013.01.003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Morales-Cabrera M, Hernández-Morales J, Leyva-Rúelas G, Salinas-Moreno Y, Soto-Rojas L, Castro-Rosas J (2013) Influence of variety and extraction solvent on antibacterial activity of roselle (Hibiscus sabdariffa L.) calyxes. J Med Plant Res 7:2319–2322. https://doi.org/10.5897/JMPR12.1242

    Article  Google Scholar 

  22. Beć K, Grabska J, Wolfgang C (2022) Chapter one - physical principles of infrared spectroscopy. Comp Anal Chem 98:1–43. https://doi.org/10.1016/bs.coac.2020.08.001

    Article  Google Scholar 

  23. Paraiso C, Santos S, Ogawa C, Sato F, Santos O, Madrona G (2020) Hibiscus sabdariffa L. extract: characterization (FTIR-ATR), storage stability and food application. Emitar J Food Agric 32:55–61. https://doi.org/10.9755/ejfa.2020.v32.i1.2059

    Article  Google Scholar 

  24. Choong Y, Nor A, Mohd W, Jamia J, Zhari I (2016) Determination of effects of sample processing on Hibiscus sabdariffa L. using tri-step infrared spectroscopy. J Anal Bioanal Tech 7:335. https://doi.org/10.4172/2155-9872.1000335

    Article  CAS  Google Scholar 

  25. Manisha S, Thilini T, Ravi S, Benu A (2021) Extraction and characterization of polyphenolic compounds and potassium hydroxycitrate from Hibiscus sabdariffa. J Future Foods 4:100087. https://doi.org/10.1016/j.fufo.2021.100087

    Article  CAS  Google Scholar 

  26. Lacaille-Dubois M, Franck U, Wagner H (2001) Search for potential angiotensin converting enzyme (ACE) inhibitors from plants. Phytomedicine 8:47–52. https://doi.org/10.1078/0944-7113-00003

    Article  CAS  PubMed  Google Scholar 

  27. Montoya-Rodríguez A, Osuna-Gallardo E, Cabrera-Chavez F, Milán-Carrillo J, Reyes-Moreno C, Milán-Noris E et al (2019) Evaluation of the in vitro and in vivo antihypertensive effect and antioxidant activity of blue corn hydrolysates derived from wet-milling. Rev Cienc Biol De La Salud XXII 155–162. https://doi.org/10.18633/biotecnia.v22i2.1257

  28. Ibnusaud I, Thomas P, Rani R, Sasi P, Beena T, Hisham A (2002) Chiral γ-butyrolactones related to optically active 2-hydroxycitric acids. Tetrahedron 58:4887–4892. https://doi.org/10.1016/s0040-4020(02)00431-3

    Article  CAS  Google Scholar 

  29. Sedillo-Torres I, Hernández-Rangel A, Gómez-y-Gómez Y, Córtes-Avalos D, García-Pérez B, Villalobos-Rocha J et al (2022) Hibiscus acid from Hibiscus sabdariffa L. inhibits flagellar motility and cell invasion in Salmonella enterica. Molecules 27:2–16. https://doi.org/10.3390/molecules27030655

    Article  CAS  Google Scholar 

  30. Natesh R, Schwager S, Evans H, Sturrock E, Acharya K (2004) Structural details on the binding of antihypertensive drugs captopril and enalaprilat. Biochem 43:8718–8724. https://doi.org/10.1021/bi049480n

    Article  CAS  Google Scholar 

  31. Martins D, Lescano C, Justo A, Vicente J, Borges A, Pires de Oliveira I, Sanjinez-Argandoña E (2023) Effect of different extraction methods on anthocyanin content in Hibiscus sabdariffa L. and their antiplatelet and vasorelaxant properties. Plant Foods Human Nutr (Dordrecht Netherlands) 78:342–350. https://doi.org/10.1007/s11130-023-01067-5

    Article  CAS  Google Scholar 

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Acknowledgements

Martinez-Ramirez E. Z gratefully acknowledge to CONACYT for the fellowship granted CVU: 845378 for Ph. D. degree.

Funding

None.

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Authors and Affiliations

  1. Química y Bioquimica de Alimentos, Tecnológico Nacional de Mexico / IT de Celaya, Campus I, Antonio Garcia Cubas Pte #600 esq. Av. Tecnologico, Celaya, C.P 38010, Mexico

    Edna Zaranne Martinez-Ramirez, Leopoldo Gonzalez-Cruz & Aurea Bernardino-Nicanor

  2. Investigador por Mexico (IxM)-TecNM en Celaya, Tecnologico Nacional de Mexico / IT de Celaya, Campus I, Antonio Garcia Cubas Pte #600 esq. Av. Tecnologico, Celaya, C.P 38010, Mexico

    Guillermo Antonio Silva-Martínez

  3. Área Académica de Química, Universidad Autónoma del Estado de Hidalgo, km. 4.5 Carretera Pachuca-Tulancingo, Ciudad del Conocimiento, Mineral de la Reforma, C.P. 42184, Hidalgo, México

    Reyna Nallely Falfan-Cortes

  4. Universidad Autónoma del Estado de Hidalgo, Mineral de la Reforma, km. 4.5 Carretera Pachuca-Tulancingo, Ciudad del Conocimiento, Mineral de la Reforma, C.P. 42184, Hidalgo, México

    Simplicio Gonzalez-Montiel & Carlos Alberto Gomez-Aldapa

Authors
  1. Edna Zaranne Martinez-Ramirez
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  2. Leopoldo Gonzalez-Cruz
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  4. Guillermo Antonio Silva-Martínez
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  6. Simplicio Gonzalez-Montiel
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  7. Carlos Alberto Gomez-Aldapa
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Contributions

EZMR, CAGA, RNAFC: Design experiment, ACE in vitro analysis approach anddiscussion of the results.ABN, LGC: FTIR analysis results and document reviewGASM, SGM: In silico analysis, MNR and document review.

Corresponding author

Correspondence to Carlos Alberto Gomez-Aldapa.

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Martinez-Ramirez, E.Z., Gonzalez-Cruz, L., Bernardino-Nicanor, A. et al. Hibiscus Acid Inhibitory Capacity of Angiotensin Converting Enzyme: an In Vitro and In Silico Study. Plant Foods Hum Nutr 79, 234–241 (2024). https://doi.org/10.1007/s11130-024-01142-5

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