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Synchronous Elicitation of Development in Root Caps Induces Transient Gene Expression Changes Common to Legume and Gymnosperm Species

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Abstract

Root cap development in cereals and legumes is self-regulated by a repressor that accumulates in the extracellular environment, and immersing the root tip into water results in renewed cap development. By exploiting this phenomenon, root cap mitosis and differentiation can be synchronously induced among populations. In Pisum sativum L., messenger RNA (mRNA) differential display revealed changes in expression of approximately 1% of the sample mRNA population within minutes of induced cap turnover. This profile changes sequentially over a period of 30 min, then stabilizes. Microarray analysis of Medicago truncatula root caps confirmed changes in expression of approximately 1% of the target population, within minutes. A cell specific marker for cap turnover exhibited the same temporal and spatial expression profile in the gymnosperm species Norway spruce (Picea abies) as in pea. Induced cap development provides a means to profile cell-specific gene expression among phylogenetically diverse species from the early moments of mitosis and cellular differentiation.

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Acknowledgment

This work was supported by funding from the Department of Energy, grant no. DE-FG02-04ER15551, and the University of Arizona College of Agriculture and Life Sciences.

We thank Dr. David Galbraith, University of Arizona, for printing of microarrays, and Dr. F.C. Gang for assistance with bioinformatics analysis.

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Authors and Affiliations

  1. Division of Plant Pathology and Microbiology, Department of Plant Sciences, University of Arizona, 303 Forbes Hall, Tucson, AZ, 85721, USA

    Fushi Wen, Ho Hyung Woo, Elizabeth A. Pierson & Martha C. Hawes

  2. Norwegian Forest and Landscape Institute, Høgskolevn. 8, 1432, Ås, Norway

    Toril D. Eldhuset, C. G. Fossdal & N. E. Nagy

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  1. Fushi Wen
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  2. Ho Hyung Woo
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  7. Martha C. Hawes
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Correspondence to Martha C. Hawes.

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Wen, F., Woo, H.H., Pierson, E.A. et al. Synchronous Elicitation of Development in Root Caps Induces Transient Gene Expression Changes Common to Legume and Gymnosperm Species. Plant Mol Biol Rep 27, 58–68 (2009). https://doi.org/10.1007/s11105-008-0058-z

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