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Biodegradation of p-hydroxybenzoic acid in soil by Pseudomonas putida CSY-P1 isolated from cucumber rhizosphere soil

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Abstract

Aims

Accumulation of p-hydroxybenzonic acid (PHBA) in soil will cause plant stress. Our aims were to characterize PHBA-degrading Pseudomonas putida CSY-P1 and to assess its role on alleviating PHBA stress in plants.

Methods

Strain CSY-P1 was isolated from rhizosphere soil, and its properties were investigated. The effects of CSY-P1 on soil enzymes and oxidative damage in plants were analyzed. Under PHBA-contaminated environments, antioxidant enzyme activities in CSY-P1 were assessed.

Results

Optimal conditions for degradation of PHBA by CSY-P1 were 28 °C, pH 9, and an initial PHBA concentration of 0.6 g l−1. Protocatechoic acid was a metabolite. CSY-P1 decomposed PHBA effectively in unplanted autoclaved soil. After the strain was applied to PHBA-contaminated cucumber-planted soil, activities of some soil enzymes were increased and the PHBA concentration in soil decreased. Some antioxidant enzyme activities in leaves were elevated, thus reducing malondialdehyde levels in seedlings and mitigating PHBA stress in cucumber. Catalase activity in PHBA-exposed CSY-P1 increased.

Conclusions

Pseudomonas putida CSY-P1 degrades PHBA in autoclaved soil and alleviates PHBA stress to plants by inducing some soil enzymes and antioxidant enzymes in leaves. Catalase in strain CSY-P1 contributes to its PHBA tolerance, making it a promising strain for remediation of PHBA-contaminated soil.

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Acknowledgments

We thank Dr. Susheng Yang (China Agricultural University, China), Dr. Binghai Du and Dr. Yanqin Ding (Shandong Agricultural University, China) for providing the Escherichia coli S17-1 strain with the pMP2444 shuttle vector.

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, Shandong, 271018, People’s Republic of China

    Shu-Yun Chen, Li-Yuan Guo, Ji-Gang Bai, Yue Zhang, Lu Zhang, Zhen Wang, Jin-Xiu Chen, Huan-Xiang Yang & Xiu-Juan Wang

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  1. Shu-Yun Chen
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  2. Li-Yuan Guo
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  3. Ji-Gang Bai
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  4. Yue Zhang
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  5. Lu Zhang
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  6. Zhen Wang
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  7. Jin-Xiu Chen
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Correspondence to Xiu-Juan Wang.

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Responsible Editor: Sven Marhan.

Shu-Yun Chen, Li-Yuan Guo and Ji-Gang Bai are contributed equally to this work.

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Chen, SY., Guo, LY., Bai, JG. et al. Biodegradation of p-hydroxybenzoic acid in soil by Pseudomonas putida CSY-P1 isolated from cucumber rhizosphere soil. Plant Soil 389, 197–210 (2015). https://doi.org/10.1007/s11104-014-2360-x

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