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Elemental 2-D mapping and changes in leaf iron and chlorophyll in response to iron re-supply in iron-deficient GF 677 peach-almond hybrid

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Abstract

Iron is an essential micronutrient for plant growth and development, involved in key cellular processes. However, the distribution of Fe in plant tissues is still not well known. In the so-called Fe chlorosis paradox, leaves of fruit trees grown in the field usually have high concentrations of Fe but still are Fe-deficient. Leaves of the Prunus rootstock GF 677 (P. dulcis × P. persica) grown in hydroponics have been used to carry out two-dimensional (2-D) nutrient mapping by synchrotron radiation-induced X-ray fluorescence. Iron-deficient leaves accumulated more Fe in the midrib and veins, with Fe concentration being markedly lower in mesophyll leaf areas. The effects of Fe deficiency and Fe re-supply on leaf chlorophyll concentration and on the distribution of Fe and other nutrients within different plant tissues have been investigated in the same plants. After Fe re-supply, leaf Fe concentrations increased largely in all leaf types. However, whereas re-greening was almost completely achieved in apical leaves, in some expanded leaves the increase in chlorophyll concentration was only moderate. Therefore, after Fe re-supply Fe-deficient expanded leaves of the Prunus rootstock GF 677 had significant increases in Fe concentration but were still chlorotic. This is similar to what occurs in leaves of peach trees in field conditions, opening the possibility that this system could be used as a model to study the Fe chlorosis paradox.

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Abbreviations

Chl:

chlorophyll

EDTA:

ethylenediaminetetracetic acid

DW:

dry weight

μ-SRXF:

synchrotron radiation-induced X-ray fluorescence

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Acknowledgements

Supported by grants from the Spanish MEC (AGL2005-05533 and AGL2006-1416) co-financed by FEDER, PETRI (PTR1995-0580) and DGA (A44 and A03). S. Jiménez was supported by an I3P-CSIC predoctoral fellowship (cofinanced by FSE). We gratefully acknowledge P. Chevallier and E. Foy for their help during the work with μ-SRXF, R. Giménez for her help with figures and A. F. López-Millán and A. Álvarez-Fernández for valuable discussion.

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Authors and Affiliations

  1. Department of Pomology, Aula Dei Experimental Station, Consejo Superior de Investigaciones Científicas (CSIC), Apdo. 13034, E-50080, Zaragoza, Spain

    S. Jiménez, M. A. Moreno & Y. Gogorcena

  2. Department of Plant Nutrition, Aula Dei Experimental Station, Consejo Superior de Investigaciones Científicas (CSIC), Apdo. 13034, E-50080, Zaragoza, Spain

    F. Morales, A. Abadía & J. Abadía

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  1. S. Jiménez
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  2. F. Morales
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  3. A. Abadía
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  4. J. Abadía
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  5. M. A. Moreno
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  6. Y. Gogorcena
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Correspondence to Y. Gogorcena.

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Responsible Editor: Jian Feng Ma.

S. Jiménez and F. Morales contributed equally to this work

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Jiménez, S., Morales, F., Abadía, A. et al. Elemental 2-D mapping and changes in leaf iron and chlorophyll in response to iron re-supply in iron-deficient GF 677 peach-almond hybrid. Plant Soil 315, 93–106 (2009). https://doi.org/10.1007/s11104-008-9735-9

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