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Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants

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Abstract

Hormone production by micro-organisms selected as antagonists of pathogenic fungi and the effect of their introduction into soil on hormone content and growth of lettuce plants were studied. Hormones in bacterial cultural media and in plant extracts were immunopurified and assayed using specific antibodies to indolyl-3-acetic acid (IAA), abscisic acid (ABA), and different cytokinins (zeatin riboside (ZR), dihydrozeatinriboside (DHZR) and isopentenyladenosine (iPA)). ZR was shown to be the main cytokinin present in bacterial cultural media as a complex with a high molecular weight component. Inoculation of lettuce plants with bacteria increased the cytokinin content of both shoots and roots. Accumulation of zeatin and its riboside was greatest in roots shortly 2 days after inoculation, when their content was 10 times higher than in control. Changes in the content of other hormones (ABA and IAA) were observed at the end of experiments only. Accumulation of cytokinins in inoculated lettuce plants was associated with an increase in plant shoot and root weight of approximately 30% over 8 days.

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Authors and Affiliations

  1. Institute of Biology of Ufa Research Centre of RAS, pr. Oktyabrya, 69, Ufa, Russia

    T. N. Arkhipova, A. I. Melentiev, E. V. Martynenko & G. R. Kudoyarova

  2. Biological Faculty, Bashkir State University, Ufa, Russia

    S. U. Veselov

Authors
  1. T. N. Arkhipova
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  2. S. U. Veselov
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  3. A. I. Melentiev
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  4. E. V. Martynenko
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  5. G. R. Kudoyarova
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Correspondence to T. N. Arkhipova.

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Arkhipova, T.N., Veselov, S.U., Melentiev, A.I. et al. Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants. Plant Soil 272, 201–209 (2005). https://doi.org/10.1007/s11104-004-5047-x

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  • DOI: https://doi.org/10.1007/s11104-004-5047-x

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