Abstract
Quinoa seeds are gluten- and cholesterol-free, contain all amino acids required by the human body, have a high protein content, provide endocrine regulation, protein supplementation, and cardiovascular protection effects. However, metabolite accumulation and transcriptional regulatory networks in quinoa seed development are not well understood. Four key stages of seed development in Dianli-3260 and Dianli-557 were thus analyzed and 849 metabolites were identified, among which sugars, amino acids, and lipids were key for developmental processes, and their accumulation showed a gradual decrease. Transcriptome analysis identified 40,345 genes, of which 20,917 were differential between the M and F phases, including 8279 and 12,638 up- and down-regulated genes, respectively. Grain development processes were mainly enriched in galactose metabolism, pentose and glucuronate interconversions, the biosynthesis of amino acids, and carbon metabolism pathways, in which raffinose, phosphoenolpyruvate, series and other metabolites are significantly enriched, gene-LOC110689372, Gene-LOC110710556 and gene-LOC110714584 are significantly expressed, and these metabolites and genes play an important role in carbohydrate metabolism, lipid and Amino acid synthesis of quinoa. This study provides a theoretical basis to expand our understanding of the molecular and metabolic development of quinoa grains.
Key message
We constructed the main network pathways of metabolites and genes during the development of quinoa grains and found that sugar and amino acids are the important metabolites.
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Acknowledgements
We gratefully acknowledge the financial support of the Yunnan Expert Workstation and Shanghai “Science and Technology Innovation Action Plan” Domestic S&T Cooperation Project. We would like to thank Wuhan MetWare Biotechnology Co., Ltd. (www.metware.cn) for metabolome and transcriptome analyses.
Funding
This work was supported by the Yunnan Expert Workstation (202205AF150001). And Shanghai “Science and Technology Innovation Action Plan” Domestic S&T Cooperation Project (22015810100).
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QW: Conceptualization, Methodology, Formal analysis, Investigation, Writing—original draft. JS: Supervision, Investigation. JL: Resources, Supervision. PZ: Investigation, Validation. LL: Investigation, Validation. HX: Investigation, Validation. HL: Supervision. HW: Methodology. CL: Methodology, Writing—review and editing. PQ: Methodology, Writing—review and editing, Funding acquisition.
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Wang, Q., Shi, J., Liu, J. et al. Integration of transcriptome and metabolome reveals the accumulation of related metabolites and gene regulation networks during quinoa seed development. Plant Mol Biol 114, 10 (2024). https://doi.org/10.1007/s11103-023-01402-z
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DOI: https://doi.org/10.1007/s11103-023-01402-z