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Genome-wide analysis of GDSL-type esterases/lipases in Arabidopsis

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Abstract

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In this present study, we introduce a fundamental framework and provide information regarding the possible roles of GDSL-type esterase/lipase gene family in Arabidopsis.

Abstract

GDSL-type esterases/lipases are hydrolytic enzymes with multifunctional properties such as broad substrate specificity, regiospecificity, and stereoselectivity. In this study, we identified 105 GDSL-type esterase/lipase genes in Arabidopsis thaliana by conducting a comprehensive computational analysis. Expression studies indicated that GDSL-type lipase proteins showed varied expression patterns. Phylogenetic tree analysis indicated that AtGELP (Arabidopsis thaliana GDSL-type esterase/lipase protein) gene family was divided into four clades. The phylogenetic analysis, combined with protein motif architectures, and expression profiling were used to predict the roles AtGELP genes. To investigate the physical roles of the AtGELP gene family, we successfully screened 88 AtGELP T-DNA knockout lines for 54 AtGELP genes from 199 putative SALK T-DNA mutants. Transgenic plants of AtGELP genes were used to elucidate the phenotypic characteristics in various developmental stages or stress conditions. Our results suggest that the AtGELP genes have diverse physical functions such as affecting the germination rate and early growth of seedlings subjected to high concentrations of glucose, or being involved in biotic stress responses.

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Acknowledgements

This research was supported by a Grant from the National Science Council of Taiwan Number NSC 102-2313-B-269-001 to C. P. Lai.

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Authors and Affiliations

  1. Department of Food and Beverage Management, Far East University, Tainan, Taiwan, Republic of China

    Chia-Ping Lai

  2. Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan, Republic of China

    Li-Min Huang, Long-Fang O. Chen, Ming-Tsair Chan & Jei-Fu Shaw

  3. Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China

    Long-Fang O. Chen

  4. Department of Biological Science and Technology, I-Shou University, Kaohsiung, Taiwan, Republic of China

    Jei-Fu Shaw

  5. Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China

    Ming-Tsair Chan & Jei-Fu Shaw

  6. Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, Taiwan, Republic of China

    Li-Min Huang & Ming-Tsair Chan

  7. Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, Republic of China

    Jei-Fu Shaw

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  1. Chia-Ping Lai
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  2. Li-Min Huang
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  5. Jei-Fu Shaw
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Contributions

CPL and LMH supervised the research design, performed research, and analyzed the data. MTC and LFOC designed research and analyzed the data. JFS supervised the study, designed research, analyzed data, and drafted the manuscript. All authors approved the final manuscript.

Corresponding author

Correspondence to Jei-Fu Shaw.

Additional information

Chia-Ping Lai and Li-Min Huang are Co-first authors.

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Lai, CP., Huang, LM., Chen, LF.O. et al. Genome-wide analysis of GDSL-type esterases/lipases in Arabidopsis . Plant Mol Biol 95, 181–197 (2017). https://doi.org/10.1007/s11103-017-0648-y

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  • DOI: https://doi.org/10.1007/s11103-017-0648-y

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