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Lipid transfer proteins in coffee: isolation of Coffea orthologs, Coffea arabica homeologs, expression during coffee fruit development and promoter analysis in transgenic tobacco plants

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Abstract

The aim of the present study was to perform a genomic analysis of non-specific lipid-transfer proteins (nsLTPs) in coffee. Several nsLTPs-encoding cDNA and gene sequences were cloned from Coffea arabica and Coffea canephora species. In this work, their analyses revealed that coffee nsLTPs belong to Type II LTP characterized under their mature forms by a molecular weight of around 7.3 kDa, a basic isoelectric points of 8.5 and the presence of typical CXC pattern, with X being an hydrophobic residue facing towards the hydrophobic cavity. Even if several single nucleotide polymorphisms were identified in these nsLTP-coding sequences, 3D predictions showed that they do not have a significant impact on protein functions. Northern blot and RT-qPCR experiments revealed specific expression of Type II nsLTPs-encoding genes in coffee fruits, mainly during the early development of endosperm of both C. arabica and C. canephora. As part of our search for tissue-specific promoters in coffee, an nsLTP promoter region of around 1.2 kb was isolated. It contained several DNA repeats including boxes identified as essential for grain specific expression in other plants. The whole fragment, and a series of 5′ deletions, were fused to the reporter gene β-glucuronidase (uidA) and analyzed in transgenic Nicotiana tabacum plants. Histochemical and fluorimetric GUS assays showed that the shorter (345 bp) and medium (827 bp) fragments of nsLTP promoter function as grain-specific promoters in transgenic tobacco plants.

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Acknowledgments

We should like to thank Gustavo Costa Rodrigues (Embrapa CNPTIA) for providing the C. arabica fruits used in this study and the members of the Apomix laboratory of Embrapa Genetic Resources and Biotechnology for assistance with microscopy. We are especially indebted to Manuel Ruiz and Jean-François Dufayard (CIRAD-UMR AGAP) for fruitful discussions and bioinformatics assistance. We are also grateful to Luciano V. Paiva and Antônio Chalfun Jr. (Lavras Federal University-UFLA) for supporting this project, to Renata H. Santana for their technical assistance and to Peter Biggins (CIRAD) for its critical reading of the manuscript. This work was funded by the Embrapa macroprogram project. The authors acknowledge the scholarships from the Brazilian agencies CNPq (Michelle G. Cotta, Eder A. Barbosa), Brazilian Consortium of Coffee Research (Felipe Vinecky, Natalia G. Vieira) and CAPES (Michelle G. Cotta, CAPES-COFECUB Project Sv738-12). The 3′ end fragment (411 bp) of the LTP promoter was patented (BR1020120081628) by INPI-Brazil.

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Authors and Affiliations

  1. Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, CP 02372, Brasília, DF, 70770-917, Brazil

    Michelle G. Cotta, Leila M. G. Barros, Juliana D. de Almeida, Eder A. Barbosa, Natalia G. Vieira, Gabriel S. C. Alves, Felipe Vinecky, Alan C. Andrade & Pierre Marraccini

  2. University of Lavras, Campus Universitário, CP 3037, Lavras, MG, 37200-000, Brazil

    Michelle G. Cotta, Natalia G. Vieira & Gabriel S. C. Alves

  3. University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, DF, 70910-900, Brazil

    Eder A. Barbosa & Felipe Vinecky

  4. INRA UMR AGAP, 34060, Montpellier, France

    Fréderic de Lamotte

  5. CIRAD UMR AGAP, 34398, Montpellier, France

    Pierre Marraccini

Authors
  1. Michelle G. Cotta
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  2. Leila M. G. Barros
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  3. Juliana D. de Almeida
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  4. Fréderic de Lamotte
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  5. Eder A. Barbosa
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  6. Natalia G. Vieira
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  7. Gabriel S. C. Alves
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  8. Felipe Vinecky
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  9. Alan C. Andrade
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  10. Pierre Marraccini
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Corresponding author

Correspondence to Pierre Marraccini.

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Cotta, M.G., Barros, L.M.G., de Almeida, J.D. et al. Lipid transfer proteins in coffee: isolation of Coffea orthologs, Coffea arabica homeologs, expression during coffee fruit development and promoter analysis in transgenic tobacco plants. Plant Mol Biol 85, 11–31 (2014). https://doi.org/10.1007/s11103-013-0166-5

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  • DOI: https://doi.org/10.1007/s11103-013-0166-5

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