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Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family

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Abstract

In hexaploid wheat, leaf rust resistance gene Lr1 is located at the distal end of the long arm of chromosome 5D. To clone this gene, an F1-derived doubled haploid population and a recombinant inbred line population from a cross between the susceptible cultivar AC Karma and the resistant line 87E03-S2B1 were phenotyped for resistance to Puccinia triticina race 1-1 BBB that carries the avirulence gene Avr1. A high-resolution genetic map of the Lr1 locus was constructed using microsatellite, resistance gene analog (RGA), BAC end (BE), and low pass (LP) markers. A physical map of the locus was constructed by screening a hexaploid wheat BAC library from cultivar Glenlea that is known to have Lr1. The locus comprised three RGAs from a gene family related to RFLP marker Xpsr567. Markers specific to each paralog were developed. Lr1 segregated with RGA567-5 while recombinants were observed for the other two RGAs. Transformation of the susceptible cultivar Fielder with RGA567-5 demonstrated that it corresponds to the Lr1 resistance gene. In addition, the candidate gene was also confirmed by virus-induced gene silencing. Twenty T 1 lines from resistant transgenic line T 0-938 segregated for resistance, partial resistance and susceptibility to Avr1 corresponding to a 1:2:1 ratio for a single hemizygous insertion. Transgene presence and expression correlated with the phenotype. The resistance phenotype expressed by Lr1 seemed therefore to be dependant on the zygosity status. T 3-938 sister lines with and without the transgene were further tested with 16 virulent and avirulent rust isolates. Rust reactions were all as expected for Lr1 thereby providing additional evidence toward the Lr1 identity of RGA567-5. Sequence analysis of Lr1 indicated that it is not related to the previously isolated Lr10 and Lr21 genes and unlike these genes, it is part of a large gene family.

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Acknowledgements

The authors extend sincere thanks to Pat Seto-Goh, Elsa Reimer, Natasa Radovanovic, Andrzej Walichnowski, Danielle Deshaies, Marcy Zirino, Tao Fan, and Mark Gray for excellent technical support throughout the project. We would also like to thank Andrzej Walichnowski for manuscript review, Joanne Schiavoni for manuscript preparation, and Michael Shillinglaw for the figures. We wish to thank CSIRO Plant Industry (Canberra, Australia) for the gift of pMNK1005. We wish to thank Large Scale Biology Corporation, Vacaville, CA for providing us with the viral vector subunits. We thank Dr. Steve Scofield for introducing us into the VIGS approach in wheat and his help setting up the system. The work on VIGS and annotation of the BAC sequences, carried out at the University of Zürich (C.L., T.W., and B.K.) was supported by the Swiss National Science Foundation (SNF) grant 3100A0–105620. This remainder of the project was carried out at the Cereal Research Centre of Agriculture and Agri-Food Canada in Winnipeg and was funded by the Canadian Crop Genomics Initiative. This is AAFC contribution number 1951.

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  1. Catherine Feuillet

    Present address: Institut National de la Recherche Agronomique, Amélioration et Santé des Plantes, 234 Ave du Brézet, 63039, Clermont Ferrand, France

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  1. Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Road, R3T 2M9, Winnipeg, MB, Canada

    Sylvie Cloutier, Brent D. McCallum, Travis W. Banks & Mark C. Jordan

  2. Institute of Plant Biology, University of Zürich, Zollikerstr. 107, 8008, Zurich, Switzerland

    Caroline Loutre, Thomas Wicker, Catherine Feuillet & Beat Keller

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Cloutier, S., McCallum, B.D., Loutre, C. et al. Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family. Plant Mol Biol 65, 93–106 (2007). https://doi.org/10.1007/s11103-007-9201-8

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