Abstract
Galanthamine, an isoquinoline alkaloid acetylcholinesterase inhibitor, is an important agent used all around the world for the symptomatic treatment of senile dementia of the Alzheimer’s type. The production of this metabolite and the availability of the plant are limited and prompted the search for an alternative way to obtain this valuable metabolite using in vitro cultures of Leucojum aestivum L. It is known that cell differentiation level shows a major influence upon the accumulation of alkaloids. For this reason, tissue cultures of L. aestivum showing different stages of morphogenesis controlled by exogenous growth regulators were established. Agrobacterium rhizogenes strain LBA 9402 has been tested for its capacity to induce hairy roots of this monocotyledonae plant.
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Bonhomme V, Laurain-Mattar D, Lacoux J, Fliniaux MA, Jacquin A (2000a) Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A,B,C genes only. J Biotechnol 81:151–158
Bonhomme V, Laurain-Mattar D, Fliniaux MA (2000b) The effects of rol C gene on hairy root induction, development and tropane alkaloid production by Atropa belladonna. J Nat Prod 63:1249–1252
Diop MF, Ptak A, Chrétien F, Henry M, Chapleur Y, Laurain-Mattar D (2006) Galanthamine content of bulbs and in vitro cultures of Leucojum aestivum L. Nat Prod Commun 1(6):475–479
Eichhorn J, Takada T, Kita Y, Zenk MH (1998) Biosynthesis of the Amaryllidaceae alkaloid galanthamine. Phytochemistry 49(4):1037–1047
Hooykaas PJJ, Shilperoort RA (1992) Agrobacterium and plant genetic engineering. Plant Mol Biol 19:15–38
Kobayashi S, Kihara M, Yuasa K, Imakura Y, Shingu T, Kato A, Hashimoto T (1985) Alkaloidal constituents of Leucojum aestivum L. (Amaryllidaceae). Chem Pharm Bull 33(12):5259–5263
Küenburg B, Czollner L, Fröhlich J, Jordis U (1999) Development of a pilot scale process for the anti-Alzheimer drug (-)-galanthamine using large scale phenolic oxidative coupling and crystallisation-induced chiral conversion. Org Process Res Dev 3:425–431
Le Flem-Bonhomme V, Laurain-Mattar D, Fliniaux MA (2004) Hairy root induction of Papaver somniferum var album, a difficult-to-transform plant, by A rhizogenes LBA 9402. Planta 218:890–893
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Palazón J, Cusido RM, Gonzalio J, Bonfill M, Morales C, Pinñol T (1998) Relation between the amount of rol Cgene product and indole alkaloid accumulation in Catharanthus roseus transformed root cultures. J Plant Physiol 153:712–718
Pinñol MT, Palazón J, Cusido R, Serrano M (1996) Effects of Ri-DNA from Agrobacterium rhizogenes on growth and hyoscyamine production in Datura stramonium root cultures. Bot Acta 109:133–138
Sellés M, Bergonon S, Viladomat F, Bastida J, Codina C (1999) Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: correlation between the state of differentiation and the content of galanthamine and related alkaloids. Plant Cell Rep 18:646–651
Stanilova MI, Ilcheva VP, Zagorska NA (1994) Morphogenic potential and in vitro micropropagation of endangered plant species Leucojum aestivumL. and Lilium rhodopaeum Delip. Plant Cell Rep 13:451–453
Trick HN, Finer JJ (1997) SAAT: sonication-assisted Agrobacterium-mediated transformation. Trans Res 6:329–336
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The authors would like to thank Bruker Daltonics for mass spectroscopy analysis.
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Diop, M.F., Hehn, A., Ptak, A. et al. Hairy root and tissue cultures of Leucojum aestivum L.—relationships to galanthamine content. Phytochem Rev 6, 137–141 (2007). https://doi.org/10.1007/s11101-006-9043-z
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DOI: https://doi.org/10.1007/s11101-006-9043-z