Photosynthetica 2013, 51(2):267-280 | DOI: 10.1007/s11099-012-0074-y

Responses to ozone on Populus "Oxford" clone in an open top chamber experiment assessed before sunrise and in full sunlight

R. Desotgiu1, M. Pollastrini1, C. Cascio1, G. Gerosa2, R. Marzuoli2, F. Bussotti1,*
1 Department of Agricultural Biotechnologies, University of Florence, Firenze, Italy
2 Department of Mathematics and Physics, Catholic University, Brescia, Italy

The effects of ambient levels of ozone and summer drought were assessed on a poplar clone (Populus maximowiczii Henry X P. × berolinensis Dippel - Oxford clone) in an open top chamber experiment carried out at the Curno facilities (Northern Italy). Chlorophyll (Chl) a fluorescence parameters (from both modulated and direct fluorescence) were assessed at different hours of the day (predawn, morning, midday, afternoon, and evening), from June to August 2008. This paper compares the results from predawn (PD, before sunrise) and afternoon (AN, in full sunlight) measurements, in order to evaluate the role of high sunlight as a factor influencing responses to ozone stress. Sunlight affected the maximum quantum yield of primary photochemistry (decrease of Fv/Fm) thus indicating photoinhibition. The effective quantum yield (ΦPSII) and the photochemical quenching (qP) were enhanced in the afternoon with respect to the predawn, whereas the nonphotochemical quenching (NPQ) was reduced. The effect of ozone was detected with fluorescence on well watered plants in the first week of July, before the onset of visible symptoms. As far as Fv/Fm are concerned, the differences between ozone-treated and control plants were statistically significant in the predawn, but not in the afternoon. Ozone exerted only minor effects on drought exposed plants because of the reduced stomatal ozone uptake, but effects on the IP phase of the fluorescence transient were observed also in drought-stressed plants.

Additional key words: fluorescence transient; JIP-test; leaf injury; linear electron transport; modulated fluorescence; drought stress; photoinhibition

Received: April 22, 2012; Accepted: September 14, 2012; Published: June 1, 2013  Show citation

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Desotgiu, R., Pollastrini, M., Cascio, C., Gerosa, G., Marzuoli, R., & Bussotti, F. (2013). Responses to ozone on Populus "Oxford" clone in an open top chamber experiment assessed before sunrise and in full sunlight. Photosynthetica, 51(2), 267-280. doi: 10.1007/s11099-012-0074-y
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