Photosynthetica 2006, 44(3):447-453 | DOI: 10.1007/s11099-006-0049-y

Structural characteristics of extra-membrane domains and guanidine hydrochloride-induced denaturation of photosystem 2 core antenna complexes CP43 and CP47

Y. G. Qu1,3, Y. D. Gong2, S. K. Guo1, L. B. Li1,*, T. Y. Kuang1,*
1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China
2 Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
3 Graduate School of the Chinese Academy of Sciences, Beijing, China

The structural characteristics of the extra-membrane domains and guanidine hydrochloride-induced denaturation of photosystem 2 (PS2) core antenna complexes CP43 and CP47 were investigated using fluorescence emission and circular dichroism (CD) spectra. The extra-membrane domains of CP43 and CP47 possessed a certain degree of secondary and tertiary structure and not a complete random coil conformation. The tertiary structure and the chlorophyll (Chl) a microenvironment of CP47 were more sensitive to guanidine hydrochloride (GuHCl) than that of CP43. Changes in energy transfer from β-carotene to Chl a corresponded well to changes in the tertiary structure while their correlation with changes in the secondary structure was rather poor. Unlike most of water-soluble proteins, both CP43 and CP47 are partly resistant to denaturation induced by guanidine hydrochloride (GuHCl); the denaturation of CP43 or CP47 is not a two-state process. Those features most probably reflect their character as intrinsic membrane proteins.

Additional key words: aromatic amino acid; chlorophyll; circular dichroism; CP43; CP47; denaturation; energy transfer; extramembrane domain; fluorescence; guanidine hydrochloride; β-carotene

Received: November 7, 2005; Accepted: January 12, 2006; Published: September 1, 2006  Show citation

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Qu, Y.G., Gong, Y.D., Guo, S.K., Li, L.B., & Kuang, T.Y. (2006). Structural characteristics of extra-membrane domains and guanidine hydrochloride-induced denaturation of photosystem 2 core antenna complexes CP43 and CP47. Photosynthetica, 44(3), 447-453. doi: 10.1007/s11099-006-0049-y
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