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Polymeric Gene Transfection on Insulin-Secreting Cells: Sulfonylurea Receptor-Mediation and Transfection Medium Effect

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Abstract

Purpose

In vitro transfection of secreting cells is regarded as one strategy for improved cell engineering/transplantation. Insulin-secreting insulinoma cell lines or pancreatic β-cells could be genetically engineered using designed polymeric vectors which are safer than viral vectors. This study investigates the effects of the constituents in transfection media on polymeric transfection.

Methods

Polyplexes conjugated with sulfonylurea (SU) were evaluated under different transfection conditions for gene transfection and their effects on cytotoxicity and insulin secretion. Several components in transfection media specifically associated with the insulin secretion pathway were amino acids, vitamins, Ca2+ and K+. The interactions of the polyplexes with insulin were monitored by surface charge and particle size to monitor how insulin as a protein influences transfection.

Results

For an insulin-secreting cell line (RINm5F), polyplexes in Ca2+-containing KRH medium (Ca2+(+)KRH) enhanced transfection and did not cause damage to biological functions. When adding amino acids, vitamins, or K+ or depleting Ca2+ from Ca2+(+)KRH, poly(l-lysine)/DNA complexes showed a greater reduction in transfection than SU receptor (SUR)-targeting polyplexes (SU-polyplex). Positively charged polyplexes interacted with insulin, developing a negative surface charge, and these interactions may cause a decrease in transfection.

Conclusion

The findings suggest that in vitro and ex vivo polymeric transfection of insulin-secreting cells can be modulated and enhanced by adjusting the transfection conditions.

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Acknowledgments

This work was partially supported by NIH DK 56884. The authors acknowledge Deepa Mishra for her proof-reading.

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Authors and Affiliations

  1. Department of Pharmaceutics and Pharmaceutical Chemistry, The University of Utah, 421 Wakara way, Suite 318, Salt Lake City, Utah, 84108, USA

    Han Chang Kang & You Han Bae

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  1. Han Chang Kang
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  2. You Han Bae
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Correspondence to You Han Bae.

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Kang, H.C., Bae, Y.H. Polymeric Gene Transfection on Insulin-Secreting Cells: Sulfonylurea Receptor-Mediation and Transfection Medium Effect. Pharm Res 23, 1797–1808 (2006). https://doi.org/10.1007/s11095-006-9027-0

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  • DOI: https://doi.org/10.1007/s11095-006-9027-0

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