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N4,N9-Dioleoyl Spermine Is a Novel Nonviral Lipopolyamine Vector for Plasmid DNA Formulation

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Purpose

To study the effect of synthesized N4,N9-dioleoyl spermine on DNA condensation and then measure its transfection efficiency in cell culture.

Methods

The lipopolyamine was synthesized from the naturally occurring polyamine spermine. The ability of this novel compound to condense DNA was studied using ethidium bromide fluorescence quenching and light scattering assays. Transfection efficiency was studied in primary skin cells (FEK4) and in an immortalized cancer cell line (HtTA), and compared with the commercially available transfection formulations Lipofectin and Lipofectamine.

Results

The synthesized N4,N9-dioleoyl spermine formula is efficient at condensing calf thymus and circular plasmid DNA and effectively transfects both primary skin cells and cancer cell lines at low charge ratios of (+/− ammonium/phosphate) 2.5.

Conclusions

N4,N9-Dioleoyl spermine condenses DNA and achieves high transfection levels in cultured cells.

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Abbreviations

DCC:

dicyclohexylcarbodiimide

DOGS:

dioctadecylamidoglycylspermine

DOSPER:

1,3-dioleoyloxy-2-(6-carboxyspermine

EGFP:

enhanced green fluorescent protein

EMEM:

Earle’s minimal essential medium

EthBr:

ethidium bromide

FCS:

fetal calf serum

HOBt:

hydroxybenzotriazole

NPC:

nuclear pore complex

PEI:

polyethylenimine

PLL:

poly-l-lysine

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Acknowledgments

We acknowledge the financial support of the Egyptian Government studentship to O.A.A. A. and Universities U.K. for an ORS award to N. A. We are grateful to Prof R.M. Tyrrell (University of Bath) for FEK4 and HtTA cell lines.

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  1. Department of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, United Kingdom

    Osama A. A. Ahmed, Noppadon Adjimatera, Charareh Pourzand & Ian S. Blagbrough

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  1. Osama A. A. Ahmed
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  2. Noppadon Adjimatera
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  4. Ian S. Blagbrough
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Correspondence to Ian S. Blagbrough.

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Ahmed, O.A.A., Adjimatera, N., Pourzand, C. et al. N4,N9-Dioleoyl Spermine Is a Novel Nonviral Lipopolyamine Vector for Plasmid DNA Formulation. Pharm Res 22, 972–980 (2005). https://doi.org/10.1007/s11095-005-4592-1

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