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Chronic Kombucha Beverage Consumption Attenuates Inflammatory Markers and Histopathology of Brain Tissue in Transnet Global Brain Ischemia in Rats

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Abstract

There is evidence that kombucha beverage (KB), a traditional fermented beverage, has a preventive effect on experimental brain ischemia. According to our previous studies, pre-treatment of KB attenuates brain edema and improves motor skills and oxidative stress in a rat model of global brain ischemia. This study was designed to evaluate the effects of the pre-treatment of KB, as a novel agent, on pro-inflammatory parameters and brain histopathology changes following global brain ischemia. Adult male Wistar rats were randomly divided into the sham, the control, and the groups treated with kombucha (KB1 and KB2 groups). KB at doses 1 and 2 mL/kg was prescribed two-week consecutive days before induction of global brain ischemia. Global brain ischemia was induced by blocking common carotid arteries for 60 min and the following reperfusion by 24 h. The serum and brain levels of tumor necrosis factor-α(TNF-α), IL-1β, histopathological change, and infarct volume are determined using the ELISA, hematoxylin and eosin (H&E), and 2,3,5-triphenyl tetrazolium chloride (TTC) staining, respectively. This study indicated that pre-treatment of KB significantly reduced infarct volume, the serum, and brain levels of TNF-α and IL-1β. The histopathological finding of the brain tissue confirmed a protective role for pre-treatment KB in the ischemic rats. Thus, the present study showed that the beneficial effects of KB pre-treatment on brain ischemic may be mediated by decreasing pro-inflammatory parameters.

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Data Availability

The datasets of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors thank Mr. Mahmud Reza Hassanzadeh for his helpful surgery assistance.

Funding

This study was financially supported by the Drug Applied Research Center of Tabriz University of Medical Sciences (Grant no. 66949), and Author F.H. has received this research support.

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Authors and Affiliations

  1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Fariba Ghiasi, Mehran Mesgari-Abbasi, Sepideh Imani & Fezzeh Hosseinzadeh

  2. Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Fariba Ghiasi

  3. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

    Monireh Khordadmehr

  4. Department of Physiology, Sarab Faculty of Medicine Sciences, Sarab, Iran

    Fezzeh Hosseinzadeh

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  1. Fariba Ghiasi
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  2. Mehran Mesgari-Abbasi
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  5. Fezzeh Hosseinzadeh
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Contributions

Fariba Ghiasi and Fezzeh Hosseinzadeh conceived and designed the study and wrote the paper. Mehran Mesgari-Abbasi, Monireh Khordadmehr, and Sepideh Imani performed laboratory experiments and contributed to the analysis. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Fezzeh Hosseinzadeh.

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There was no conflict of interest.

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The study was approved by the research and ethics committee of Tabriz University of Medical Sciences in Iran (the ethics code was (IR.TBZMED.VCR.REC.1400.115). All procedures were performed according to guidelines for the care and use of laboratory animals at Tabriz University of Medical Sciences.

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Ghiasi, F., Mesgari-Abbasi, M., Khordadmehr, M. et al. Chronic Kombucha Beverage Consumption Attenuates Inflammatory Markers and Histopathology of Brain Tissue in Transnet Global Brain Ischemia in Rats. Neurochem Res 48, 3202–3211 (2023). https://doi.org/10.1007/s11064-023-03980-2

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