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LncRNA H19 Attenuates Apoptosis in MPTP-Induced Parkinson’s Disease Through Regulating miR-585-3p/PIK3R3

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Abstract

Parkinson’s disease (PD) is a prevalent age-related neurodegenerative disease which is modulated by various molecules, including long non-coding RNAs (lncRNAs). LncRNA H19 has been shown to be associated with PD progression, but the mechanism is still unclear. This research aims to investigate the role of H19 in PD development and the detailed mechanisms. Our results showed that H19 was down-regulated in brain tissue of MPTP-induced PD mice (in vivo) and in MPP+ treated human neuroblastoma cells. miR-585-3p was verified to be a target of lncRNA H19 and was negatively regulated by H19. In addition, H19 could increase the expression of PIK3R3 through miR-585-3p. In vitro results indicated that H19 inhibited the apoptosis of MPP+ treated neuroblastoma cells by regulating of miR-585-3p. Moreover, in PD model mice, overexpression of H19 attenuated MPTP-induced neuronal apoptosis. In summary, our present research demonstrated that LncRNA H19 could attenuate neurons apoptosis in MPTP-induced PD mice as well as MPP+ treated neuroblastoma cells through regulating miR-585-3p/PIK3R3. The results may provide a potential theoretical experimental data for the clinical treatment of PD through targeting lncRNAs or miRNAs.

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All data generated or analyzed during this study are included in this published article.

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Authors and Affiliations

  1. Department of Neurosurgery, Guizhou People’s Hospital, No. 83, Zhongshang East Road, Nanming District, Guiyang, 550002, Guizhou, China

    Yong Zhang, Qiming Xia & Jun Lin

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  1. Yong Zhang
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  2. Qiming Xia
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  3. Jun Lin
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Contributions

JL conceived and designed the experiments,YZ analyzed and interpreted the results of the experiments, QM performed the experiments.

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Correspondence to Jun Lin.

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The authors state that there are no conflicts of interest to disclose.

Ethical Approval

All the procedure was in accordance with the Guide for the Care and Use of Laboratory Animals and approved by the Animal Ethics Committee of Guizhou People’s Hospital(Approval No. 2019-009).

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Zhang, Y., Xia, Q. & Lin, J. LncRNA H19 Attenuates Apoptosis in MPTP-Induced Parkinson’s Disease Through Regulating miR-585-3p/PIK3R3. Neurochem Res 45, 1700–1710 (2020). https://doi.org/10.1007/s11064-020-03035-w

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  • DOI: https://doi.org/10.1007/s11064-020-03035-w

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