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Comparison of Immunoreactivities of Calbindin-D28k, Calretinin and Parvalbumin in the Striatum Between Young, Adult and Aged Mice, Rats and Gerbils

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Abstract

Calcium binding proteins play important roles in all aspects of neural functioning in the central nervous system. In the present study, we examined age-related changes of three different calcium binding proteins calbindin-D28k (CB), calretinin (CR) and parvalbumin (PV) immunoreactivities in the striatum of young (1 month), adult (6 months) and aged (24 months) ages in three species of rodents (mouse, rat and gerbil) using immunohistochemistry and Western blotting. Our results show that the number of CB-immunoreactive neurons was highest in the adult mouse and rat; however, in the gerbil, the number of CB-immunoreactive neurons was not significantly different from each group although the CB immunoreactivity was significantly decreased in the aged group compared with the adult group. The number of CR-immunoreactive neurons in the striatum was significantly highest in all the adult groups, and, especially, the number of CR-immunoreactive neurons and CR immunoreactivity in the aged gerbil were significantly decreased in the aged group compared with the other groups. Finally, we did not found any significant difference in the number of PV-immunoreactive neurons in the striatum with age among the three rodents. On the other hand, we found that protein levels of three calcium binding proteins in all the mouse groups were similar to the immunohistochemical data. These results indicate that the distribution pattern of calcium binding proteins is different according to age; the adult might show an apparent tendency of high expression in the striatum.

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Acknowledgments

The authors would like to thank Mr. Seung Uk Lee for his technical help in this study. This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0010580), and by a Priority Research Centers Program Grant (NRF-2009-0093812) through the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning.

Conflict of interest

The authors declare that they have no conflict of interest.

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    Authors and Affiliations

    1. Department of Pediatrics, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon, 200-701, South Korea

      Eun Joo Bae

    2. Department of Physiology, Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea

      Bai Hui Chen, Bich Na Shin & Yun Lyul Lee

    3. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

      Jeong Hwi Cho, In Hye Kim, Joon Ha Park, Jae Chul Lee, Moo-Ho Won & Ji Hyeon Ahn

    4. Department of Biomedical Science, Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, 200-702, South Korea

      Hyun Jin Tae & Soo Young Choi

    5. Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, 200-701, South Korea

      Jong-Dai Kim

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    1. Eun Joo Bae
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    Correspondence to Moo-Ho Won or Ji Hyeon Ahn.

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    Eun Joo Bae and Bai Hui Chen have contributed equally to this article.

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    Bae, E.J., Chen, B.H., Shin, B.N. et al. Comparison of Immunoreactivities of Calbindin-D28k, Calretinin and Parvalbumin in the Striatum Between Young, Adult and Aged Mice, Rats and Gerbils. Neurochem Res 40, 864–872 (2015). https://doi.org/10.1007/s11064-015-1537-x

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    • DOI: https://doi.org/10.1007/s11064-015-1537-x

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