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The Expression Patterns of Nogo-A, Myelin Associated Glycoprotein and Oligodendrocyte Myelin Glycoprotein in the Retina After Ocular Hypertension

The Expression of Myelin Proteins in the Retina in Glaucoma

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Abstract

Nogo-A, a major myelin inhibitory protein, inhibits axon growth and synaptic function in the central nervous system. Glaucoma is a progressive neuropathy as a result of retinal ganglion cell (RGC) death. Synaptic degeneration is thought to be an early pathology of neurodegeneration in glaucoma and precedes RGC loss. Here experimental ocular hypertension model was induced in adult rats with laser coagulation of the episcleral and limbal veins. The expression of Nogo-A, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp) in the retina was investigated using immunohistochemistry and Western blotting. We found that Nogo-A was expressed in the RGCs and upregulated after the induction of ocular hypertension. OMgp was only expressed in the inner plexiform layer. There was no MAG expression in the retina. Our data provided, for the first time, the expression patterns of three myelin proteins in the adult retina and suggested an important role of Nogo-A in the RGC death and synaptic degeneration in glaucoma.

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Acknowledgments

This study was supported by funding from the Jessie Ho Professorship in Neurosciences from the University of Hong Kong, NSFC (30801272, 81071030), Science and Technology Foundation of Guangdong Province, China (2008B030301090, 2010B031600089), RFDP (200805581160), the Fundamental Research Funds for the Central Universities (09ykpy25, 09ykpy31) and National Basic Research Program of China (973 Program) (2011CB707501).

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Authors and Affiliations

  1. Department of Cardiovasology, Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China

    Xin-Xue Liao

  2. Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, 510080, Guangdong, China

    Dong Chen, Jianbo Shi, Yue-Qi Sun, Shu-Juan Sun & Qing-Ling Fu

  3. Department of Anatomy, The State Key Laboratory of Brain and Cognitive Sciences, and the Research Centre of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China

    Kwok-Fai So & Qing-Ling Fu

  4. Joint Laboratory for Brain Function and Health (BFAH), Jinan University and The University of Hong Kong, Guangzhou, 510632, Guangdong, China

    Kwok-Fai So

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  1. Xin-Xue Liao
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  2. Dong Chen
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Correspondence to Qing-Ling Fu.

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Liao XX and Chen D contributed equally to this work.

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Liao, XX., Chen, D., Shi, J. et al. The Expression Patterns of Nogo-A, Myelin Associated Glycoprotein and Oligodendrocyte Myelin Glycoprotein in the Retina After Ocular Hypertension. Neurochem Res 36, 1955–1961 (2011). https://doi.org/10.1007/s11064-011-0518-y

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