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Strain Difference in the Up-Regulation of FGF-2 Protein Following a Neurotoxic Lesion of the Nigrostriatal Pathway

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Abstract

Lesions of the nigrostriatal pathway are known to induce a compensatory up-regulation of various neurotrophic factors. In this study we examined protein content of basic fibroblast growth factor (FGF-2) in tissue samples taken from the ventral midbrain and striatum at two different time points following a neurotoxic lesion of the nigrostriatal pathway in two different rat strains, the outbred Sprague–Dawley (SD) and inbred F344 × Brown Norway F1 hybrid (F344BNF1). Despite both rat strains having comparable lesions of the nigrostriatal pathway, we observed a difference in the temporal up-regulation of FGF-2 in ventral midbrain samples taken from the side ipsilateral to the lesion. Basic FGF was significantly up-regulated in ventral midbrain in SD rats 1 week post-lesion while we did not observe an up-regulation of FGF-2 in the lesioned ventral midbrain of F344BNF1 at this same time point. However, both strains showed a significant up-regulation of FGF-2 in the lesioned ventral midbrain 3 weeks post-lesion. Sprague–Dawley rats also appeared to be more sensitive to the lesion in terms of up-regulating FGF-2 expression. The differences reported here suggest currently unknown genetic differences between these two strains may be important factors for regulating the compensatory release of neurotrophic factors, such as FGF-2, in response to a neurotoxic lesion of the nigrostriatal pathway.

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Acknowledgments

This research was supported by DOD grant DAMD 17-01-1-0786 (DMY) and NIH grants NS50311 (DMY) and AG17963 (WAC).

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Authors and Affiliations

  1. Department of Neurosurgery, University of Kentucky College of Medicine, Health Sciences Research Building, Lexington, KY, 40536-0305, USA

    David M. Yurek & Anita M. Fletcher

  2. Department of Anatomy & Neurobiology, University of Kentucky College of Medicine, Lexington, KY, 40536, USA

    Laura E. Peters & Wayne A. Cass

Authors
  1. David M. Yurek
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  2. Anita M. Fletcher
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  3. Laura E. Peters
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  4. Wayne A. Cass
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Correspondence to David M. Yurek.

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Yurek, D.M., Fletcher, A.M., Peters, L.E. et al. Strain Difference in the Up-Regulation of FGF-2 Protein Following a Neurotoxic Lesion of the Nigrostriatal Pathway. Neurochem Res 35, 531–539 (2010). https://doi.org/10.1007/s11064-009-0093-7

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  • DOI: https://doi.org/10.1007/s11064-009-0093-7

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