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Astrocytic Expressions of Phosphorylated Akt, GSK3β and CREB Following an Excitotoxic Lesion in the Mouse Hippocampus

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Abstract

Glycogen synthase kinase 3β (GSK3β) is believed to play important roles in the regulation of synaptic plasticity, cell survival and circadian rhythms in the mature CNS. However, although several studies have been focused on the GSK3β, little is known about GSK3β changes in glial cells under neuropathological conditions. In this study, we evaluated the expressions of molecules associated with the GSK3β signaling pathway, following the induction of an excitotoxic lesion in mouse brain by kainic acid (KA) injection, which caused pyramidal cell degeneration in the hippocampal CA3 region. In injured hippocampi, Ser47-Akt (protein kinase B, PKB) phosphorylation increased from 4 h until 1 day post-injection (PI). Ser9-GSK3β and Ser133-cAMP responsive element-binding protein (CREB) phosphorylations showed similar spatiotemporal patterns in hippocampi at 1 day until 3 days PI. Double immunohistochemistry also showed that these phosphorylated forms of Akt, GSK3β and CREB were expressed in astrocytes. For the first time, our data demonstrate the injury-induced astrocytic changes in the levels of phosphorylation of Akt, -GSK3β and -CREB in vivo, which may reflect mechanisms of glial cells protection or adaptive response to damage.

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Acknowledgements

This study was supported by a grant (M103KV010018 04K2201 01850) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, the Republic of Korea and in part by grant no 800-20060228 from the Seoul National University Hospital Research Fund.

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Authors and Affiliations

  1. Department of Anatomy, Chungnam National University College of Medicine, Daejeon, 301-131, South Korea

    Dong Woon Kim, Woo-Mi Yang & Young Ho Lee

  2. Department of Anatomy, Seoul National University College of Medicine, Yongon-Dong, Chongno-Gu, Seoul, 110-799, South Korea

    Jung Hoon Lee, Sung Kyung Park, Gye Sun Jeon & Sa Sun Cho

  3. Department of Neurosurgery, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, 110-744, South Korea

    Chun Kee Chung

  4. MRC Neuroscience Institute, Seoul National University College of Medicine, Seoul, 110-799, South Korea

    Sa Sun Cho

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  1. Dong Woon Kim
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  2. Jung Hoon Lee
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Correspondence to Sa Sun Cho.

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DW Kim and JH Lee contributed equally to this work.

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Kim, D.W., Lee, J.H., Park, S.K. et al. Astrocytic Expressions of Phosphorylated Akt, GSK3β and CREB Following an Excitotoxic Lesion in the Mouse Hippocampus. Neurochem Res 32, 1460–1468 (2007). https://doi.org/10.1007/s11064-007-9332-y

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  • DOI: https://doi.org/10.1007/s11064-007-9332-y

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