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Nicotine Inhibits bFGF-induced Neurite Outgrowth through Suppression of NO Synthesis in H19-7 Cells

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Abstract

NO (Nitric oxide) has been known as a biological signaling molecule that can function as a beneficial agent in physiologically essential functions such as differentiation or neurotransmission. In this study, we elucidated how nicotine inhibits neuronal differentiation induced by the basic fibroblast growth factor (bFGF) in hippocampal cell line, H19-7 cells, because nicotine is one of the key neuroregulatory components. Treatment of H19-7 cells with bFGF increased NO production through upregulated iNOS/nNOS expression, and also increased expressions of neuronal markers such as brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3) and Neuro-D. Pretreatment of the cells with nicotine decreased iNOS promoter activity as well as iNOS/nNOS expression induced by bFGF, resulting in decreased NO production. Nicotine also suppressed expressions of BDNF, NT3 and Neuro-D, resulting in decreased bFGF-induced neurite outgrowth. These results indicate that nicotine inhibits bFGF-induced neuronal differentiation in H19-7 cells through inhibition of NO formation by suppressing iNOS/nNOS expressions.

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Acknowledgments

This research was largely supported by a grant from Korea Research Foundation (015-E00070,2004) and partly by a grant from Research Center for Bio-Medicinal Resources (Bio-Med RRC), PaiChai University.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Hanyang university, 17 Haengdang-dong, Sungdong-gu, Seoul, 133-791, Republic of Korea

    Shin-Young Park, Ju Hwan Cho, Doo-Yi Oh & Joong-Soo Han

  2. Department of Pediatrics, College of Medicine, Hanyang University, Seoul, 133-791, Korea

    Young Jae Koh, Su-A Shin & Ha-Baik Lee

  3. The Research Center for Bio-Medicinal Resourses, PaiChai University, Taejon, 302-735, Korea

    Ki-Sung Lee & Joong-Soo Han

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  1. Shin-Young Park
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  2. Young Jae Koh
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Correspondence to Joong-Soo Han.

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Park, SY., Koh, Y.J., Cho, J.H. et al. Nicotine Inhibits bFGF-induced Neurite Outgrowth through Suppression of NO Synthesis in H19-7 Cells. Neurochem Res 32, 481–488 (2007). https://doi.org/10.1007/s11064-006-9256-y

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  • DOI: https://doi.org/10.1007/s11064-006-9256-y

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