We studied the processes of proliferation and apoptosis in different zones of the cerebellum in juvenile individuals of the parr (masu) salmon, Oncorhynchus masou, after experimental mechanical trauma; we took into account that the brain of young fishes of this species is in the state of development, and that this is accompanied by intense proliferative activity in the matrix zones. In the damaged cerebellum, results of TUNEL labeling demonstrated manifestations of high apoptotic activity in both zones of postembryonic neurogenesis (granular eminences and molecular layer) and the superficial layer of the cerebellum body. The reaction to mechanical injury of the cerebellum looked like post-traumatic intensifications of proliferative activity in the matrix zones and regional neurogenic niches combined with intensification of the processes of apoptosis, which reached the maximum in the zones of radial migration of the cells. This, obviously, provided elimination of some cells formed due to both reparative neurogenesis and natural morphogenetic processes. In the injured region, we observed manifestations of tangential and radial migration of the cells. A high value of the apoptosis index was observed in the dorsal part of the cerebellum, which corresponded to the zone of secondary neurogenesis. Therefore, apoptotic elimination of some cells is realized already at the stage of formation of the latter in the matrix zones. Apoptotic reactions in the regions where the background morphogenetic processes and processes related to physiological reparation after a traumatic influence prevail and in the remote regions are characterized by dissimilar intensities.
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References
G. K. Zupanc, «Neurogenesis and neuronal regeneration in the adult fish brain,” J. Comp. Physiol., 192, 649-670 (2006).
G. K. Zupanc, U. M. Wellbrock, R. F. Sîrbulescu, et al., “Generation, long-term persistence, and neuronal differentiation of cells with nuclear aberrations in the adult zebrafish brain,” Neuroscience, 159, 1338-1348 (2009).
R. W. Williams, “Mapping genes that modulate brain development: a quantitative genetic approach,” Mouse Brain Dev., 30, 21-49 (2000).
A. Takeda, M. Nakano, R. C. Goris, et al., “Adult neurogenesis with 5-HT expression in lesioned goldfish spinal cord,” Neuroscience, 151, 1132-1114 (2008).
G. K. Zupanc and R. F. Sîrbulescu, “Teleost fish as a model system to study successful regeneration of the central nervous system,” Curr. Topics Microbiol. Immunol., 367, 193-233 (2013).
G. K. Zupanc, S. C. Clint, N. Takimoto, et al., «Spatiotemporal distribution of microglia/macrophages during regeneration in the cerebellum of adult teleost fish, Apteronotus leptorhynchus: a quantitative analysis,” Brain, Behav., Evolut., 62, 31-42 (2003).
E. Pouwels, “On the development of the cerebellum of the trout, Salmo gairdneri: I. Patterns of cell migration,” Anat. Embryol., 152, 291-308 (1978).
G. A. Merculov, Course of Pathohistological Technique [in Russian], Leningrad (1969).
B. Romeis, Microscopic Technique [Russian translation], Izd. Inostr. Lit., ино стр. лит., Moscow (1953).
G. K. Zupanc, “Neurogenesis, cell death and regeneration in the adult gymnotiform brain,” J. Exp. Biol., 202, 1435-1446 (1999).
N. G. Andreyeva and D. K. Obukhov, Evolutionary Morphology of the Nervous System of Vertebrates [in Russian], Lan, Saint Petersburg (1999).
E. Сandal, R. Anadon, F. Bourrat, et al., «Cell proliferation in the developing and adult hindbrain and midbrain of trout and medaka (teleosts): A segmental approach,» J. Dev. Brain Res., 160, 157-175 (2005).
G. K. Zupanc and R. Ott, «Cell proliferation after lesions in the cerebellum of adult teleost fish: time course, origin, and type of new cells produced,» Exp. Neurol., 160, 78-87 (1999).
Ye. V. Pushchina, D. K. Obukhov, and A. A. Varaksin, “Neurochemical markers of cells of the periventricular brain area in the masu salmon Oncorhynchus masou (Salmonidae),” Russ. J. Dev. Biol., 43, No. 1, 35-48 (2012).
K. Nagamoto-Combs, D. W. McNeal, R. J. Morecraft, et al., “Prolonged microgliosis in the rhesus monkey central nervous system after traumatic brain injury,” J. Neurotrauma, 24, 1719-1742 (2007).
R. S. Rajendran, M. M Zupanc, and A. Lösche, “Numerical chromosome variation and mitotic segregation defect in the adult brain of teleost fish,” Dev. Neurobiol., 67, 1334-1347 (2007).
P. Ekström, C. M. Johnsson, and L. M. Ohlin, “Ventricular proliferation zones in the brain of an adult teleost fish and their relation to neuromeres and migration (secondary matrix) zones,” J. Comp. Neurol., 436, 92-110 (2001).
E. V. Puschina, D. K. Obukhov, and A. A. Varaksin, «Features of adult neurogenesis and neurochemical signaling in the Cherry salmon Oncorhynchus masou brain,” Neural. Regen. Res., 8, No. 1, 13-23 (2013).
E. V. Puschina and D. K. Obukhov, «Processes of proliferation and apoptosis in the brain of the Amur Sturgeon,» Neurophysiology, 43, No. 4, 271-286 (2011).
G. K. Zupanc and M. M. Zupanc, “Proteomic analysis of CNS injury and recovery,” Аdv. Neurobiol., 2, 511-536 (2011).
S. G. Kalinichenko and N. Yu. Matveyeva, «Morphological properties of apoptosis and its importance in neurogenesis,” Morfologiya, 131, 16-28 (2007).
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Stukaneva, M.E., Puschina, E.V. Processes of Proliferation and Apoptosis in the Cerebellum of the Parr (Oncorhynchus masou) after Mechanical Injury. Neurophysiology 47, 376–385 (2015). https://doi.org/10.1007/s11062-016-9545-2
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DOI: https://doi.org/10.1007/s11062-016-9545-2