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Expression of Genes for Temperature-Sensitive TRP Channels in the Rat Hypothalamus in Normal Conditions and on Adaptation to Cold

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Quantitative RT-PCR was used to study the expression of genes for temperature-sensitive TRP (transient receptor potential) ion channels in the hypothalamus in control (kept for five weeks at +20 ± 22°C) and cold-adapted (five weeks at +4 ± 6°C) rats. We report the first observation of expression of the genes Trpv3 and Trpm8, evidencing the presence of TRPV3 and TRPM8 ion channels in the hypothalamus; the expression of the genes for six temperature-sensitive TRP ion channels (TRPA1, TRPM8, TRPV1, TRPV2, TRPV3, and TRPV4) was compared, and quite high levels of expression of the genes for temperature-sensitive ion channels activated at temperatures exceeding 30°C were noted, while the levels of expression of genes for cold-sensitive TRPM8 and TRPA1 were much lower; changes in the expression of the gene for TRPV3 channels due to cold adaptation were seen. This latter point supports the suggestion that temperature-sensitive TRP ion channels in the hypothalamus are involved in the mechanism of temperature adaptation at the genome level.

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Authors and Affiliations

  1. Institute of Physiology, Siberian Branch, Russian Academy of Medical Sciences, 4 Academician Timakov Street, 630117, Novosibirsk, Russia

    I. P. Voronova, A. A. Tuzhikova & T. V. Kozyreva

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  1. I. P. Voronova
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  2. A. A. Tuzhikova
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  3. T. V. Kozyreva
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Correspondence to I. P. Voronova.

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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 98, No. 9, pp. 1101–1110, September, 2012.

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Voronova, I.P., Tuzhikova, A.A. & Kozyreva, T.V. Expression of Genes for Temperature-Sensitive TRP Channels in the Rat Hypothalamus in Normal Conditions and on Adaptation to Cold. Neurosci Behav Physi 44, 565–570 (2014). https://doi.org/10.1007/s11055-014-9952-z

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