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Superparamagnetic nano-immunobeads toward food safety insurance

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Abstract

In this work, superparamagnetic nano-immunobeads (SPM-NIBs) based on conjugation of superparamagnetic Fe3O4 nanoparticles with specific antibodies have been developed toward food safety insurance. The resultant SPM-NIBs exhibits excellent colloidal stability and reversible magnetic response. Vibrio parahaemolyticus, which is a main foodborne pathogenes from contaminated seafood, can be separated specifically and efficiently by the resultant SPM-NIBs. The results of bacteria separation demonstrate that the SPM-NIBs have a higher specific activity and sensitivity toward V. parahaemolyticus. About 80 % of V. parahaemolyticus cells can be captured when the concentration of the broth reaches 103 CFU/mL. Thus, the SPM-NIBs can effectively enhance the efficiency for target bacteria inspections by shortening the period of culture time. This work holds the promise of development of general technique to prepare effective SPM-NIBs toward food safety inspections and other bio-related applications for target analyte separation and collection.

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Acknowledgments

We gratefully acknowledge financial support from the Quality Inspection Non-profit Industry Special Research Project (201110034).

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Authors and Affiliations

  1. National Center for Nanoscience and Technology, No. 11, Beiyitiao, Zhongguancun, Beijing, 100190, People’s Republic of China

    Xuefeng Liu, Yan Gao & Zhiyong Tang

  2. Beijing Entry-Exit Inspection and Quarantine Bureau, No. 6, Tianshuiyuan Street, Beijing, 100026, People’s Republic of China

    Lei Zhang & Jing Zeng

Authors
  1. Xuefeng Liu
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  2. Lei Zhang
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  3. Jing Zeng
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  4. Yan Gao
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  5. Zhiyong Tang
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Correspondence to Jing Zeng or Yan Gao.

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Liu, X., Zhang, L., Zeng, J. et al. Superparamagnetic nano-immunobeads toward food safety insurance. J Nanopart Res 15, 1796 (2013). https://doi.org/10.1007/s11051-013-1796-x

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  • DOI: https://doi.org/10.1007/s11051-013-1796-x

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