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Lysosomal diacylglycerol pyrophosphate phosphatase is not essential in Trypanosoma brucei

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Abstract

Mg2+-independent phosphatidic acid phosphatase (PAP2), diacylglycerol pyrophosphate phosphatase 1 (Dpp1) is a membrane-associated enzyme in Saccharomyces cerevisiae. The enzyme is responsible for inducing the breakdown of β-phosphate from diacylglycerol pyrophosphate (DGPP) into phosphatidate (PA) and then removes the phosphate from PA to give diacylglycerol (DAG). In this study through RNAi suppression, we have demonstrated that Trypanosoma brucei diacylglycerol pyrophosphate phosphatase 1 (TbDpp1) procyclic form production is not required for parasite survival in culture. The steady-state levels of triacylglycerol (TAG), the number of lipid droplets, and the PA content are all maintained constant through the inducible down-regulation of TbDpp1. Furthermore, the localization of C-terminally tagged variants of TbDpp1 in the lysosome was demonstrated by immunofluorescence microscopy.

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No data associated in the manuscript.

Abbreviations

TAG:

Triacylglycerol

DG:

Diacylglycerol

DGPP:

Diacylglycerol Pyrophosphate

DPP1:

Diacylglycerol Pyrophosphate Phosphatase 1

FBS:

Fetal Bovine Serum

LPP:

Lipid Phosphate Phosphatase 1

PA:

Phosphatidic Acid

PAk:

Phosphatidate Kinase

PAP:

Phosphatidic Acid Phosphatase

PLD:

Phospholipase D

RNAi:

RNA interference

TLC:

Thin-Layer Chromatography

CatL:

Cathepsin L

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Acknowledgements

We appreciate Dr. Peter Bütikofer’s guidance and support. L.D.N thanks PE. Aryan for encouragement. The work was supported by the Swiss National Science Foundation grants 31003 A-169355 to P.B.

Funding

The work was supported by the Swiss National Science Foundation grants 31003 A-169355 to Peter Bütikofer. Ladan Dawoody Nejad has received research support from the Swiss National Science Foundation grants.

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Authors and Affiliations

  1. Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC, Canada

    Ladan Dawoody Nejad

  2. Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland

    Ladan Dawoody Nejad

  3. Graduate School for Cellular and Biochemical Sciences, University of Bern, Bern, Switzerland

    Ladan Dawoody Nejad

  4. Department of Translational Biomedicine and Neuroscience, University of Bari, Bari, Italy

    Tiziana Annese & Domenico Ribatti

  5. Department of Medicine and Surgery, Libera Università del Mediterraneo (LUM) Giuseppe Degennaro, Bari, Italy

    Tiziana Annese

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  1. Ladan Dawoody Nejad
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Material preparation, data collection and analysis were performed by Ladan Dawoody Nejad. The first draft of the manuscript was written by Ladan Dawoody Nejad and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ladan Dawoody Nejad.

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Dawoody Nejad, L., Annese, T. & Ribatti, D. Lysosomal diacylglycerol pyrophosphate phosphatase is not essential in Trypanosoma brucei. Mol Biol Rep 51, 578 (2024). https://doi.org/10.1007/s11033-024-09547-w

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