Skip to main content

Advertisement

SpringerLink
Log in

Cardamonin decreases inflammatory mediator expression in IL-1β-stimulated human periodontal ligament cells

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Background

Cardamonin is classified as a natural chalcone, and has been reported to possess various bioactive effects. However, there have been limited attempts to utilize cardamonin in the treatment of periodontitis. This study aimed to investigate whether cardamonin has anti-inflammatory effects on human periodontal ligament cells (HPDLCs), which are a component cell of periodontal tissue. Specifically, the study seeks to determine whether cardamonin affects the expression of inflammatory mediators, such as cytokines and adhesion molecules, induced by interleukin-1β (IL-1β) in HPDLCs, as well as the signaling pathways activated by IL-1β.

Methods

Cytokine and chemokine levels in supernatants of HPDLCs were measured by ELISA. Western blot analysis was used to measure protein expression and signal transduction pathway activation in HPDLCs.

Results

We found that IL-1β-induced CC chemokine ligand (CCL)2, CCL5, CCL20, CXC-chemokine ligand (CXCL)10, and interleukin (IL)-6 production and intercellular adhesion molecule (ICAM)-1 and cyclooxygenase (COX)-2 expression in HPDLCs were suppressed by cardamonin treatment. We also found that cardamonin suppressed IL-1β-activated nuclear factor (NF)-κB pathway, and the phosphorylation of signal transducer and activator of transcription (STAT)3. Furthermore, cardamonin treatment enhanced the expression of the antioxidant enzymes, heme oxygenase (HO)-1 and NAD(P)H dehydrogenase [quinone] 1 (NQO1), in HPDLCs.

Conclusion

In this study, we found that cardamonin could suppress the production of inflammatory mediators in HPDLCs as well as the activation of several signaling pathways induced by IL-1β treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Data availability

The data in this manuscript are available from the corresponding author upon request.

References

  1. Graves D (2008) Cytokines that promote periodontal tissue destruction. J Periodontol 79:1585–1591

    Article  PubMed  CAS  Google Scholar 

  2. Ng E, Tay JRH, Boey SK, Laine ML, Ivanovski S, Seneviratne CJ (2023) Antibiotic resistance in the microbiota of periodontitis patients: an update of current findings. Crit Rev Microbiol 4:1–12

    Article  Google Scholar 

  3. Nawaz J, Rasul A, Shah MA, Hussain G, Riaz A, Sarfraz I, Zafar S, Adnan M, Khan AH, Selamoglu Z (2020) Cardamonin: a new player to fight cancer via multiple cancer signaling pathways. Life Sci 250:117591

    Article  PubMed  CAS  Google Scholar 

  4. Israf DA, Khaizurin TA, Syahida A, Lajis NH, Khozirah S (2007) Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-kappaB nuclear translocation and Ikappa-B phosphorylation in RAW 264.7 macrophage cells. Mol Immunol 44:673–679

    Article  PubMed  CAS  Google Scholar 

  5. Yu H, Jiang W, Du H, Xing Y, Bai G, Zhang Y, Li Y, Jiang H, Zhang Y, Wang J, Wang P, Bai X (2014) Involvement of the Akt/NF-κB pathways in the HTNV-mediated increase of IL-6, CCL5, ICAM-1, and VCAM-1 in HUVECs. PLoS ONE 9:e93810

    Article  PubMed  PubMed Central  Google Scholar 

  6. Barber K, Mendonca P, Evans JA, Soliman KFA (2023) Antioxidant and anti-inflammatory mechanisms of Cardamonin through Nrf2 activation and NF-kB suppression in LPS-Activated BV-2 microglial cells. Int J Mol Sci 24:10872

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. Trivedi S, Lal N (2017) Antioxidant enzymes in periodontitis. J Oral Biol Craniofac Res 7:54–57

    Article  PubMed  Google Scholar 

  8. Araujo JA, Zhang M, Yin F (2012) Heme oxygenase-1, oxidation, inflammation, and atherosclerosis. Front Pharm 3:119

    Article  Google Scholar 

  9. Atia A, Alrawaiq N, Abdullah A (2014) A review of NAD(P)H: quinone oxidoreductase 1(NQO1); a multifunctional antioxidant enzyme. J App Pharm Sci 4:118–122

    Google Scholar 

  10. Hosokawa I, Hosokawa Y, Ozaki K, Matsuo T (2020) Carnosic acid inhibits inflammatory cytokines production in human periodontal ligament cells. Immunopharmacol Immunotoxicol 42:373–378

    Article  PubMed  CAS  Google Scholar 

  11. Hosokawa Y, Hosokawa I, Shindo S, Ohta Y, Ozaki K, Matsuo T (2016) Alkannin inhibits CCL3 and CCL5 production in human periodontal ligament cells. Cell Biol Int 40:1380–1385

    Article  PubMed  CAS  Google Scholar 

  12. Joe BH, Borke JL, Keskintepe M, Hanes PJ, Mailhot JM, Singh BB (2001) Interleukin-1beta regulation of adhesion molecules on human gingival and periodontal ligament fibroblasts. J Periodontol 72:865–870

    Article  PubMed  CAS  Google Scholar 

  13. Kato T, Segami N, Sakagami H (2016) Anti-inflammatory activity of Hangeshashinto in IL-1β-stimulated Gingival and Periodontal ligament fibroblasts. In Vivo 30:257–263

    PubMed  CAS  Google Scholar 

  14. Nilsson BO (2021) Mechanisms involved in regulation of periodontal ligament cell production of pro-inflammatory cytokines: implications in periodontitis. J Periodontal Res 56:249–255

    Article  PubMed  CAS  Google Scholar 

  15. Ambili R, Janam P, Saneesh Babu PS, Prasad M, Vinod D, Anil Kumar PR, Kumary TV, Asha Nair S (2017) Differential expression of transcription factors NF-κB and STAT3 in periodontal ligament fibroblasts and gingiva of healthy and diseased individuals. Arch Oral Biol 82:19–26

    Article  PubMed  CAS  Google Scholar 

  16. Sczepanik FSC, Grossi ML, Casati M, Goldberg M, Glogauer M, Fine N, Tenenbaum HC (2020) Periodontitis is an inflammatory disease of oxidative stress: we should treat it that way. Periodontol 2000 84:45–68

    Article  PubMed  Google Scholar 

  17. Isola G, Santonocito S, Lupi SM, Polizzi A, Sclafani R, Patini R, Marchetti E (2023) Periodontal Health and Disease in the Context of Systemic Diseases. Mediators Inflamm. 2023:9720947

  18. Hosokawa Y, Hosokawa I, Shindo S, Ozaki K, Matsuo T (2015) Calcitriol suppressed inflammatory reactions in IL-1β-Stimulated Human Periodontal Ligament cells. Inflammation 38:2252–2258

    Article  PubMed  CAS  Google Scholar 

  19. Peng YJ, Lu JW, Lee CH, Lee HS, Chu YH, Ho YJ, Liu FC, Huang CJ, Wu CC, Wang CC (2021) Cardamonin attenuates inflammation and oxidative stress in Interleukin-1β-Stimulated osteoarthritis chondrocyte through the Nrf2 pathway. Antioxidants 10:862

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  20. Arce M, Rodriguez-Peña M, Espinoza-Arrue J, Godoy RA, Reyes M, Kajikawa T, Greenwell-Wild T, Hajishengallis G, Abusleme L, Moutsopoulos N, Dutzan N (2023) Increased STAT3 activation in Periodontitis drives inflammatory bone loss. J Dent Res. https://doi.org/10.1177/00220345231192381

    Article  Google Scholar 

  21. Chen YC, Cheng YK, Chen JH, Tsai CF, Wang TK, Wu CY, Chang PC, Yeh WL (2022) Cardamonin attenuates phorbol 12-myristate 13-acetate-induced pulmonary inflammation in alveolar macrophages. Food Chem Toxicol 159:112761

    Article  PubMed  CAS  Google Scholar 

  22. Jing W, Liu C, Su C, Liu L, Chen P, Li X, Zhang X, Yuan B, Wang H, Du X (2023) Role of reactive oxygen species and mitochondrial damage in rheumatoid arthritis and targeted drugs. Front Immunol 14:1107670

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  23. Sahoo DK, Heilmann RM, Paital B, Patel A, Yadav VK, Wong D, Jergens AE (2023) Oxidative stress, hormones, and effects of natural antioxidants on intestinal inflammation in inflammatory bowel disease. Front Endocrinol 14:1217165

    Article  Google Scholar 

Download references

Funding

This research was supported by Grant-in-Aid for Scientific Research (C) under Grants 22K09966 and 22K09984 from the Japan Society for the Promotion of Science and Research, and the cluster program of Tokushima University (Grant Number: 2202006).

Author information

Authors and Affiliations

  1. Department of Regenerative Dental Medicine, Tokushima University Graduate School of Biomedical Sciences, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan

    Risa Okamoto, Yoshitaka Hosokawa, Ikuko Hosokawa & Keiichi Hosaka

  2. Department of Oral Health Care Promotion, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Tokushima, Japan

    Kazumi Ozaki

  3. Division of Interdisciplinary Research for Medicine and Photonics, Institute of Post LED Photonics, Tokushima University, Tokushima, Tokushima, Japan

    Keiichi Hosaka

Authors
  1. Risa Okamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoshitaka Hosokawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ikuko Hosokawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kazumi Ozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Keiichi Hosaka
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Conceptualization, YH; investigation, RO, YH, and IH; methodology, RO, YH, IH, and KO; writing—original draft, RO and YH; writing—review and editing, KO and KH. The published version of the manuscript has been read and approved by all authors.

Corresponding author

Correspondence to Yoshitaka Hosokawa.

Ethics declarations

Ethical approval

This article does not include any studies involving human subjects or animals that were conducted by any of the by any of the authors.

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Okamoto, R., Hosokawa, Y., Hosokawa, I. et al. Cardamonin decreases inflammatory mediator expression in IL-1β-stimulated human periodontal ligament cells. Mol Biol Rep 51, 222 (2024). https://doi.org/10.1007/s11033-023-09204-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11033-023-09204-8

Keywords

Search

Navigation