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Provitamin A, lysine and tryptophan enrichment in shrunken2-based sweet corn genotypes through genomics-assisted breeding for crtRB1 and opaque2 genes

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Abstract

Background

Malnutrition affects large section of population worldwide. Vitamin A and protein deficiencies have emerged as the major global health-issue. Traditional shrunken2 (sh2)-based sweet corn is deficient in provitamin A (proA), lysine and tryptophan. Natural variant of β-carotene hydroxylase1 (crtRB1) and opaque2 (o2) enhances proA, lysine and tryptophan in maize. So far, no sweet corn hybrid rich in these nutrients has been released elsewhere. Development of biofortified sweet corn hybrids would help in providing the balanced nutrition.

Methods and Results

We targeted three sh2-based sweet corn inbreds (SWT-19, SWT-20 and SWT-21) for introgression of mutant crtRB1 and o2 genes using molecular breeding. The gene-based 3′TE-InDel and simple sequence repeat (SSR) (umc1066) markers specific to crtRB1 and o2, respectively were utilized in foreground selection in BC1F1, BC2F1 and BC2F2. Segregation distortion was observed for crtRB1 and o2 genes in majority of populations. Background selection using 91–100 SSRs revealed recovery of recurrent parent genome (RPG) up to 96%. The introgressed progenies possessed significantly higher proA (13.56 µg/g) as compared to the original versions (proA: 2.70 µg/g). Further, the introgressed progenies had accumulated moderately higher level of lysine (0.336%) and tryptophan (0.082%) over original versions (lysine: 0.154% and tryptophan: 0.038%). Kernel sweetness among introgressed progenies (17.3%) was comparable to original sweet corn (17.4%). The introgressed inbreds exhibited higher resemblance with their recurrent parents for yield and morphological characters.

Conclusion

These newly developed biofortified sweet corn genotypes hold immense promise to alleviate malnutrition.

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Data availability

All the data sets supporting the conclusion of this article are included within the article, and its supporting information files are provided as accompanying supplementary materials.

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Acknowledgements

We thank WNC, IIMR, Hyderabad for providing the off-season nursery.

Funding

We are thankful to the ICAR-IARI, New Delhi for financial support.

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Authors and Affiliations

  1. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Bhavna Singh, Rajkumar U. Zunjare, Gulab Chand, Nisrita Gain, Vinay Bhatt, Vignesh Muthusamy & Firoz Hossain

  2. Amity Institute of Biotechnology, Amity University, Noida, India

    Bhavna Singh & Smriti Shrivastava

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  1. Bhavna Singh
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Contributions

Conduct of the experiment: BS, Generation of backcross populations: RUZ, Recording of phenotypic data: GC and VB, Estimation of quality parameters: NG, Statistical analysis: VM, Writing of manuscript: BS, GC and FH, Designing of experiment: FH and SS.

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Correspondence to Firoz Hossain.

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Singh, B., Zunjare, R.U., Shrivastava, S. et al. Provitamin A, lysine and tryptophan enrichment in shrunken2-based sweet corn genotypes through genomics-assisted breeding for crtRB1 and opaque2 genes. Mol Biol Rep 50, 4965–4974 (2023). https://doi.org/10.1007/s11033-023-08446-w

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