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The protective effects of resveratrol on ulcerative colitis via changing the profile of Nrf2 and IL-1β protein

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Abstract

Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) with increasing incidence and prevalence in developed countries. The presence of inflammatory cytokines is considered the main detrimental factor in severe types of IBD. The Nrf2 transcription factor plays an important role in reducing the expression of inflammatory agents such as interleukin (IL)-1β and increasing reparative factors such as IL-11. Resveratrol, a plant-derived phenolic compound, reduces the damage in chronic experimentally induced colitis. Twenty patients with UC and also 20 healthy controls were recruited in this study. The proteins expression of Nrf2 and IL-1β was assessed in colonic biopsies by Western blotting. Caco-2 cells were challenged with TNF-α (in vitro simulation of UC), in the presence or not of 190 nM (24 h) and 75 nM (48 h) Resveratrol. Then, Nrf2 and IL-1β in gene and protein expression were measured by real time-PCR and Western blotting in different treatments. Finally, IL-11 proteins expression was measured in culture supernatant by ELISA. A significant increase of IL-1β protein was detected in inflamed colonic tissues from UC patients compared with the control individuals. In Caco-2 cells challenged with TNF-α, protein expression of IL-1β and p-Nrf2 showed an increase, while gene expression of Nrf2 did not show a significant difference. After treatment with Resveratrol, both IL-1β mRNA and protein levels were reduced, while IL-11 protein levels showed any increase. The p-Nrf2 is a dominant form which is prevalent in inflamed tissues from UC patients. Resveratrol can reverse the inflammatory effects of TNF-α by reducing IL-1β and increasing IL-11 production.

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Acknowledgements

The authors are grateful to the staff of Cellular & Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran, and the authorities of the endoscopy unit of Shahrekord Hajar Hospital, Shahrekord, Iran, for their cooperation.

Funding

This work was supported by the National Institute for Medical Research Development (Nimad), Tehran, Iran, with Grant Number of 971537.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Milad Sabzevary-Ghahfarokhi

  2. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Amin Soltani

  3. Department of Health Sciences, University of Catanzaro “Magna Graecia”, 88100, Catanzaro, Italy

    Francesco Luzza & Tiziana Larussa

  4. Department of Internal Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Ghorbanali Rahimian

  5. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Hedayatollah Shirzad & Nader Bagheri

Authors
  1. Milad Sabzevary-Ghahfarokhi
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  2. Amin Soltani
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  3. Francesco Luzza
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  4. Tiziana Larussa
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  5. Ghorbanali Rahimian
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  6. Hedayatollah Shirzad
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  7. Nader Bagheri
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Correspondence to Nader Bagheri.

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Authors declare no conflict of interest.

Ethical approval

The Ethical Board of Shahrekord University of Medical Sciences supported this work (Number IR.SKUMS.REC.1395.313). The aim of the study was explained to each study individuals. They were informed that personal data were not published. All patients signed written informed consent to donate their tissue samples for research.

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Sabzevary-Ghahfarokhi, M., Soltani, A., Luzza, F. et al. The protective effects of resveratrol on ulcerative colitis via changing the profile of Nrf2 and IL-1β protein. Mol Biol Rep 47, 6941–6947 (2020). https://doi.org/10.1007/s11033-020-05753-4

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  • DOI: https://doi.org/10.1007/s11033-020-05753-4

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