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Analysis of CRABP2 and FABP5 genes in primary and recurrent pterygium tissues

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Abstract

The etiology of pterygium remains unclear, but ultraviolet (UV) radiation is generally considered to be major risk factor. Pterygium has similarity features with many cancers, including inflammation, invasion, cell proliferation, anti-apoptosis, angiogenesis and recurrence after resection. Retinoic acid via cellular retinoic acid binding protein 2 (CRABP2) is involved in cell cycle arrest, apoptosis and differentiation, while it via fatty acid binding protein 5 (FABP5) is involved in survival, cell proliferation and angiogenesis, which pathway gets activated depends on the CRABP2/FABP5 ratio. Alterations of retinoid signaling were found in many cancer types. The deregulated retinoid signaling may also contribute to the development and/or recurrence of pterygium. The aim of our study was to determine mRNA and protein expressions of CRABP2 and FABP5 and ratio of CRABP2/FABP5 in primer and recurrent pterygium tissues. Pterygia tissues were collected from 30 eyes of 30 patients undergoing pterygium excision. CRABP2 and FABP5 mRNA and protein expression were assessed using Real-time PCR and Western blotting through examination of excised specimens from pterygium and conjunctiva tissues. The ratio of CRABP2/FABP5 gene expression was not altered when primary pterygium tissues compared normal conjunctival tissues (1.00-fold change). Whereas the ratio of CRABP2/ FABP5 gene expression was decreased when recurrent pterygium tissues compared normal conjunctival tissues (0.81-fold change). Understanding etiopathogenesis of pterygium may aid in the find of more promising treatments to prevent pterygium in earlier stages.

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Acknowledgements

The authors thank Selim DEMİR and Helin DENİZ DEMİR for their help in obtaining the patient’s samples.

Funding

This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) 1001 (Grant Number: 215S692).

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Authors and Affiliations

  1. Medical Faculty, Department of Medical Biology, Gaziosmanpasa University, 60100, Tokat, Turkey

    Sumeyya Deniz Celik & Omer Ates

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  1. Sumeyya Deniz Celik
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Contributions

Concept: SDC, OA; Data Collection and/or Processing: SDC, OA; Interpretation: SDC, OA; Genetic analyzing: SDC, OA; Literature Review: SDC; Writer: SDC; Critical Review: SDC, OA. The authors thank Selim DEMİR and Helin DENİZ DEMİR for their help in obtaining the patient’s samples.

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Correspondence to Sumeyya Deniz Celik.

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Celik, S.D., Ates, O. Analysis of CRABP2 and FABP5 genes in primary and recurrent pterygium tissues. Mol Biol Rep 47, 6105–6110 (2020). https://doi.org/10.1007/s11033-020-05686-y

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  • DOI: https://doi.org/10.1007/s11033-020-05686-y

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