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Development and characterization of twelve microsatellite loci for Amolops ricketti using next-generation sequencing

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Abstract

Amphibian populations have decreased dramatically because of human disturbance. To better conserve and manage native or wild populations of Amolops ricketti, 12 novel microsatellite loci were developed using high-throughput sequencing. The number of alleles ranged from 2 to 19, with an average of 7.67 alleles per locus. The observed and expected heterozygosity ranged from 0.42 to 1.00 (average 0.81) and from 0.51 to 0.95 (average 0.76), respectively. The polymorphism information content per locus ranged from 0.38 to 0.92 (average 0.69). These polymorphic microsatellite markers will contribute to the assessment of population structure, gene flow and genetic differentiation in A. ricketti.

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Acknowledgments

This work was supported by Zhejiang Provincial Natural Science Foundation of China (L19C030005, LQ14C040001) and the National Natural Science Foundation of China (31400472).

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Authors and Affiliations

  1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, China

    Ning Wei, Xia Kan, Chen Shao & Yu Wang

  2. Key Laboratory of Wildlife Biotechnology, Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua, China

    Yu Wang

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  1. Ning Wei
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  2. Xia Kan
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  3. Chen Shao
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  4. Yu Wang
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Correspondence to Yu Wang.

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The authors declare that they have no conflict of interest.

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This research was approved by the Gutian Mountain National Nature Reserve Authority and Ethics Committee of Zhejiang Normal University, and it was conducted under Law of the People’s Republic of China on the Protection of Wildlife (August 28, 2004).

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Wei, N., Kan, X., Shao, C. et al. Development and characterization of twelve microsatellite loci for Amolops ricketti using next-generation sequencing. Mol Biol Rep 47, 6435–6437 (2020). https://doi.org/10.1007/s11033-020-05654-6

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  • DOI: https://doi.org/10.1007/s11033-020-05654-6

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