Skip to main content
SpringerLink
Log in

Genetic diversity in Corchorus olitorius L. revealed by morphophysiological and molecular analyses

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Assessment of genetic diversity has an efficient role in plant breeding and improvement programs. There is a limit number of investigations dealing with the evaluation of genetic diversity in Jew’s mallow (Corchorus olitorius L.), despite its valuable importance as a leafy vegetable and a delicious dish rich in vitamins and antioxidants. Therefore, in this study, 18 landraces of Jew’s mallow—collected from different locations in Egypt—were used for genetic diversity assessment based on morphophysiological and molecular evaluations. A high degree of variability was found among the evaluated landraces at both levels, indicating the appropriateness of such collection to be involved in breeding approaches. Some morphophysiological traits offered a high level of diversity and effectively discriminated the landraces. Thus, they are recommended to be used in successive morphological evaluation studies. On the other hand, molecular evaluation using the random amplified polymorphic DNA (RAPD) and the sequence related amplified polymorphism (SRAP) efficiently supported the morphological results by exposing a clear genetic relationship among the landraces. In addition, the principal coordinate analysis based on combined data of RAPD and SRAP divided the landraces into two main groups, reflecting their relationship molecularly. The first group included nine landraces related to Upper Egypt and the second gathered three landraces from Delta, while the other six landraces were distinctly distributed around these two groups. The two groups may have two distinct ancestors in addition to the different ancestors of the scattered landraces. Findings of this study are valuable and could assist in Jew’s mallow breeding programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Heywood VH, Brummitt RK, Culham A, Seberg O (2007) Flowering plant families of the world. Royal Botanic Gardens, Kew

    Google Scholar 

  2. Mir RR, Rustgi S, Sharma S, Singh R, Goyal A, Kumar J, Gaur A, Tyagi AK, Khan H, Sinha MK, Balyan HS, Gupta PK (2007) A preliminary genetic analysis of fiber traits and the use of new genomic SSRs for genetic diversity in jute. Euphytica 161:413–427

    Article  CAS  Google Scholar 

  3. Hillocks RJ (1998) The potential benefits of weeds with reference to small holder agriculture in Africa. Integr Pest Manag Rev 3:155–167

    Article  Google Scholar 

  4. Pal DK, Mandal M, Senthilkumar GP, Padhiari A (2006) Antibacterial activity of Cuscuta reflexa stem and Corchorus olitorius seed. Fitoterapia 77:589–591

    Article  CAS  PubMed  Google Scholar 

  5. Mavengahama S, McLachlan M, Clercq W (2013) The role of wild vegetable species in household food security in maize based subsistence cropping systems. Food Sci 5:227–233

    Article  Google Scholar 

  6. Azuma KN, Masayoshi KM, Ippoushi KY, Yuichi KK, Yamauchi YI, Hidekazu HH (1999) National research institute of vegetables, ornamental plants and tea, Ano, Mie, 514–2392. J Agric Food Chem 47(47):3963–3966

    Article  CAS  PubMed  Google Scholar 

  7. Heikal HA, Abd El-Azeem RH, El-Wakil HE (2015) Molecular fingerprinting and phylogenetic relationships among three Egyptian Moulokhyia genotypes (Corchorus olitorius L.) using RAPD, ISSR and SRAP markers. Indian Streams Res J 5(1):1–12

    Google Scholar 

  8. Youssef M, Ibrahim RA, Amein KA (2015) Comparison of phenotypic and molecular assessment of genetic diversity in guava. Acta Hortic 1100:115–120

    Article  Google Scholar 

  9. Hossain MB, Haque S, Khan H (2002) DNA fingerprinting of jute germplasm by RAPD. J Biochem Mol Biol 35(4):414–419

    CAS  PubMed  Google Scholar 

  10. Ognkanmi LA, Okunowo WO, Oyelakin OO, Oboh BO, Adesina OO, Adekoya KO, Ogundipe OT (2010) Assessment of genetic relationships between two species of jute plants using phenotypic and RAPD markers. Int J Bot 6(2):107–111

    Article  Google Scholar 

  11. Chen Y, Zhang L, Qi J, Chen H, Tao A, Xu J, Lin L, Fang P (2014) Genetic linkage map construction for white jute (Corchorus capsularis L.) using SRAP, ISSR and RAPD markers. Plant Breed 133:777–781

    Article  CAS  Google Scholar 

  12. Benor S, Demissew S, Hammer K, Blattner FR (2012) Genetic diversity and relationships in Corchorus olitorius (Malvaceae s.l.) inferred from molecular and morphological data. Genet Resour Crop Evol 59:1125–1146

    Article  CAS  Google Scholar 

  13. Huq S, Islam S, Sajib AA, Ashraf N, Haque S, Khan H (2009) Genetic diversity and relationships in jute (Corchorus spp.) revealed by SSR markers. Bangladesh J Bot 38(2):153–161

    Article  Google Scholar 

  14. Chen H, Chen M, Tao A, Zhang G, Xu J, Qi J, Fang P (2011) Construction of a molecular linkage map using SRAP and mapping of three qualitative traits in Corchorus olitorius L. Sci Agric Sin 44(12):2422–2430

    CAS  Google Scholar 

  15. Carter MR, Gregorich EG (2008) Soil sampling and methods of analysis, 2nd edn. CRC Press, Boca Raton, p 1224

    Google Scholar 

  16. Hassan AA (1991) Production of vegetable crops, 1st edn. Arab House for Publishing and Distribution, Cairo, (in Arabic)

    Google Scholar 

  17. Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382

    Article  CAS  Google Scholar 

  18. Nissen O (1984) MSTAT. A microcomputer program for statistical analyses of experiments and surveys. In: Riley H, Skjelvag AO (eds) The impact of climate on grass production and quality. In: Proceedings of general meeting of European Grassland Federation, 10th, As, Norway. 26–30 June 1984. Norwegian State Agric Res Stations, As, pp 555–559

  19. Youssef M, Valdez-Ojeda R, Ku-Cauich JR, Escobedo-GraciaMedrano R (2015) Enhanced protocol for isolation of plant genomic DNA. J Agric Environ Sci 4:172–180

    Google Scholar 

  20. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461

    Article  CAS  Google Scholar 

  22. Roldan-Ruiz I, Dendauw J, VanBockstaele E, Depicker A, De Loose M (2000) AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol Breed 6:125–134

    Article  CAS  Google Scholar 

  23. Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98:107–112

    Article  CAS  Google Scholar 

  24. Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York

    Book  Google Scholar 

  25. Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

    Google Scholar 

  26. Maina FNW, Reuben MM, Gohole LS (2012) Morphological characterization of jute mallow, Corchorus sp. to assess its genetic diversity in western Kenya. Baraton Interdiscip Res J 2(2):21–29

    Google Scholar 

  27. Denton OA, Nwangburuka CC (2012) Morphological diversity among Corchorus olitorius accessions based on single linkage cluster analysis and principal component analysis. Jordan J Biol Sci 5(3):191–196

    Google Scholar 

  28. Adebo HO, Ahoton LE, Quenum F, Ezin V (2015) Agro-morphological characterization of Corchorus olitorius cultivars of benin. Annu Res Rev Biol 7(4):229–240

    Article  Google Scholar 

  29. Adeyemo O, Abati A (2015) Genetic diversity in Corchorus Olitorius L. grown in South-West Nigeria inferred from RAPD data. Jordan J Agric Sci 11(2):329–337

    Google Scholar 

  30. Ghosh RK, Wongkaew A, Sreewongchai T, Nakasathien S, Phumichai C (2014) Assessment of genetic diversity and population structure in jute (Corchorus spp.) using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Kasetsart J Nat Sci 48:83–94

    CAS  Google Scholar 

  31. Nag S, Mitra J, Satya P, Kar CS, Karmakar PG (2018) Genetic diversity assessment in jute (Corchorus species) utilizing inter simple sequence repeat and simple sequence repeat markers. Indian J Biotechnol 17:316–326

    CAS  Google Scholar 

  32. Das M, Banerjee S, Topdar N, Kundu A, Sarkar D, Sinha MK, Balyan HS, Gupta PK (2011) Development of large-scale AFLP markers in jute. J Plant Biochem Biotechnol 20(2):270–275

    Article  Google Scholar 

  33. Hamza AH, El-Wakil HE, Khaled AE, Fayed A, Abdelsalam NR, Hebatullah MR (2013) Biochemical and molecular studies on three local genotypes of jew’s mallow (Corchorus olitorius L.). J Adv Agric Res 18(4):30–47

    Google Scholar 

  34. Cruz-Cárdenas CI, Youssef M, Escobedo-Graciamedrano RM (2017) Assessment of genetic relationship in Musa using male flower descriptors and molecular markers. S Afr J Bot 113:270–276

    Article  CAS  Google Scholar 

  35. Ferriol M, Pico B, Nuez F (2003) Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor Appl Genet 107:271–282

    Article  CAS  PubMed  Google Scholar 

  36. Youssef M, James AC, Rivera-Madrid R, Ortiz R, Escobedo-GraciaMedrano RM (2011) Musa genetic diversity revealed by SRAP and AFLP. Mol Biotechnol 47:189–199

    Article  CAS  PubMed  Google Scholar 

  37. Youssef M, Elazab DS (2015) Molecular analysis of Ziziphus spp. cultivars in Egypt. Acta Hortic 1100:37–44

    Article  Google Scholar 

Download references

Acknowledgements

This work was conducted at Department of Horticulture, Faculty of Agriculture, Al-Azhar University, Assiut Branch and Genetics Departments, Faculty of Agriculture, Assiut University, Egypt.

Author information

Authors and Affiliations

  1. Department of Horticulture, Faculty of Agriculture, Al-Azhar University-Assiut - Branch, Assiut, Egypt

    Ahmed Fathy Youssef & Nabil Ahmed Younes

  2. Department of Genetics, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt

    Muhammad Youssef

Authors
  1. Ahmed Fathy Youssef
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nabil Ahmed Younes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Muhammad Youssef
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Muhammad Youssef.

Ethics declarations

Conflict of interest

None of the authors have any financial or non-financial conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 13 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Youssef, A.F., Younes, N.A. & Youssef, M. Genetic diversity in Corchorus olitorius L. revealed by morphophysiological and molecular analyses. Mol Biol Rep 46, 2933–2940 (2019). https://doi.org/10.1007/s11033-019-04754-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-019-04754-2

Keywords

Search

Navigation